Proteins toxic to hemipteran insect species
The present invention discloses Hemipteran insect inhibitory proteins, methods of using such proteins, nucleotide sequences encoding such proteins, methods of detecting and isolating such proteins, and their use in agricultural systems.
1. An insect inhibitory recombinant polypeptide comprising the amino acid sequence as set forth in SEQ ID NO: 16.
2. The insect inhibitory recombinant polypeptide of claim 1 , wherein the polypeptide exhibits inhibitory activity against an insect species of the order Hemiptera.
3. The insect inhibitory recombinant polypeptide of claim 2 , wherein the Hemipteran species is selected from the group consisting of a Lygus sp., an Emrasca sp., and an Amrasca sp.
4. The insect inhibitory recombinant polypeptide of claim 2 , wherein the Hemipteran species is selected from the group consisting of Lygus Hesperus, Lygus lineolaris , and Amrasca devastans.
5. A polynucleotide encoding the insect inhibitory recombinant polypeptide of claim 1 .
6. The polynucleotide of claim 5 , wherein the polynucleotide comprises the nucleotide sequence as set forth in SEQ ID NO: 15.
7. A host cell comprising the polynucleotide of claim 5 , wherein the host cell is selected from the group consisting of a bacterial host cell and a plant host cell.
8. The host cell of claim 7 , wherein the bacterial host cell is selected from the group consisting of uii Agrobacterium, Rhizobium, Bacillus, Escherichia, Pseudomonas, and Salmonella ; and
wherein the Bacillus species is a Bacillus thuringiensis , and the Escherichia is an Escherichia coli.
9. The host cell of claim 7 , wherein the plant host cell is selected from the group consisting of a monocot cell and a dicot cell.
10. An insect inhibitory composition comprising the insect inhibitory recombinant polypeptide of claim 1 .
11. The composition of claim 10 , wherein the composition is prepared by lyophilization, extraction, filtration, or centrifugation.
12. The insect inhibitory composition of claim 10 , further comprising at least one insect inhibitory agent different from the insect inhibitory recombinant polypeptide.
13. The insect inhibitory composition of claim 12 , wherein the at least one insect inhibitory agent is selected from the group consisting of an insect inhibitory protein, an insect inhibitory dsRNA molecule, and an insect inhibitory chemistry.
14. The insect inhibitory composition of claim 12 , wherein the at least one insect inhibitory agent exhibits activity against one or more pest species of the order Lepidoptera, Coleoptera, Hemiptera, or Homoptera.
15. A seed comprising the polynucleotide of claim 5 .
16. The seed of claim 15 , wherein the polynucleotide comprises the sequence as set forth in SEQ ID NO: 15.
17. A method of controlling a Hemipteran pest, the method comprising presenting the Hemipteran pest with an inhibitory amount of the insect inhibitory recombinant polypeptide of claim 1 .
18. The method of claim 17 , wherein said presenting is via expressing the insect inhibitory recombinant polypeptide in a cotton plant.
19. A transgenic plant cell, plant, or plant part comprising the insect inhibitory recombinant polypeptide of claim 1 .
20. A method of controlling a Hemipteran pest, comprising exposing the Hemipteran pest to the transgenic plant cell, plant or plant part of claim 19 , wherein the plant cell, plant or plant part expresses a Hemipteran inhibitory amount of the insect inhibitory recombinant polypeptide.
21. A commodity product obtained from the plant cell, plant, or plant part of claim 19 , wherein the commodity product comprises a detectable amount of the insect inhibitory recombinant polypeptide, wherein the commodity product is selected from the group consisting of plant biomass, oil, meal, animal feed, flour, flakes, bran, lint, hulls, and processed seed.
22. A method of producing a seed, the method comprising:
a. planting at least one seed comprising a polynucleotide encoding the insect inhibitory recombinant polypeptide of claim 1 ;
b. growing at least one plant from the at least one seed; and
c. harvesting a seed from the at least one plant, wherein the harvested seed comprises the polynucleotide encoding the insect inhibitory recombinant polypeptide.