IP Library › Granted Patent US 12,427,521
Granted Patent B2
US 12,427,521 · App. 18/595,920 · Granted Sep 30, 2025

Sorting particles in a microfluidic device

Inventors: Ravi Kapur (Sharon, MA); Kyle C. Smith (Cambridge, MA); Mehmet Toner (Charlestown, MA)
Assignee: The General Hospital Corporation
B01L3/502761A61K35/28B01L3/502715B01L3/502746B01L3/502753G01N1/4077G01N15/0255G01N15/0618G01N15/1484B01L3/502776B01L2200/0631B01L2200/0647B01L2200/0652B01L2200/0668B01L2200/0684B01L2200/12B01L2300/0681B01L2300/0877B01L2300/185B01L2400/0409B01L2400/0415B01L2400/043B01L2400/0436B01L2400/0457B01L2400/0487B01L2400/082G01N2001/4088G01N2015/0053G01N15/01G01N2015/0288G01N2015/1486G01N15/149G01N2015/1493
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Quick Facts
Patent No.
US 12,427,521
App. No.
18/595,920
Granted
Sep 30, 2025
Kind
B2
Abstract

A microfluidic device includes a particle sorting region having a first, second and third microfluidic channels, a first array of islands separating the first microfluidic channel from the second microfluidic channel, and a second array of islands separating the first microfluidic channel from the third microfluidic channel, in which the island arrays and the microfluidic channels are arranged so that a first fluid is extracted from the first microfluidic channel into the second microfluidic channel and a second fluid is extracted from the third microfluidic channel into the first microfluidic channel, and so that particles are transferred from the first fluid sample into the second fluid sample within the first microfluidic channel.

Claims (10)

1. A microfluidic device comprising:

a module comprising an array of islands in a microfluidic channel, a buffer inlet in fluid communication with a first end of the microfluidic channel, and a product inlet in fluid communication with a first product outlet and a first end of the microfluidic channel, wherein:

the array of islands is arranged in one or more rows that extend along a longitudinal direction in a corresponding microfluidic channel,

each island in a row is spaced apart from an adjacent island in the row to form a siphoning channel, and

the array of islands is configured and arranged to shift portions of fluid through the siphoning channel between adjacent islands within a row to a waste outlet, and to shift particles above a specific size into a buffer flowing in the microfluidic channel and to a second product outlet.

2. The microfluidic device of claim 1 , wherein the arrays of islands include four arrays of islands.

3. The microfluidic device of claim 2 , wherein the four arrays of islands are each arranged in parallel such that a different portion of a sample fluid introduced into the microfluidic device flows through each of the four arrays of islands.

4. The microfluidic device of claim 1 , wherein each island included in the array of islands has a width between 150 and 250 micrometers, a length between 200 and 800 micrometers, and a height between 100 and 200 micrometers.

5. The microfluidic device of claim 1 , wherein each island included in the array of islands has a length-to-width ratio greater than 1.25.

6. The microfluidic device of claim 1 , wherein the siphoning channels formed in the array of islands have a respective channel width of 50 micrometers.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 11, 2024
From: SMITH, KYLE C.; KAPUR, RAVI; TONER, MEHMET
To: THE GENERAL HOSPITAL CORPORATION
Reel/Frame 066711/0465 →
Continuity (7)
Continuation 17245770 · Apr 30, 2021
Continuation 16112259 · Aug 24, 2018
Continuation 15891579 · Feb 8, 2018
Division 14931223 · Nov 3, 2015
Provisional Application 62074213 · Nov 3, 2014
Provisional Application 62074315 · Nov 3, 2014
Related Publication 20240198338A1 · Jun 20, 2024
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