Method and apparatus for sorting particles using asymmetrical particle shifting
An apparatus and method disperse particles suspended in a fluid with an obstacle field in the flow path of the fluid. The particles may be dispersed after an interaction with obstacles in the obstacle field. The obstacle-particle interactions may result in an asymmetrical particle shift in which the particles are dispersed in an asymmetrical manner relative to the obstacle and the fluid flow. Obstacles are arranged to separate particles flowing through the device based on individual obstacles having properties that are asymmetrical and are oriented and aligned for the separation.
1. A device for interacting with particles in a fluid, the device comprising:
an inner lumen; and
a plurality of obstacles disposed in at least a portion of the inner lumen, wherein the obstacles are arranged to separate particles flowing through the device, wherein at least a portion of the individual obstacles each have an asymmetrical property that causes at least some of the particles in the fluid to shift asymmetrically about the individual obstacles with the asymmetrical properties, wherein the asymmetrical properties are oriented and aligned for the separation of the particles flowing through the device.
2. The device of claim 1 , wherein the asymmetrical properties result in different magnitudes of particle shift on either side of an asymmetrical obstacle.
3. The device of claim 1 , wherein the asymmetrical properties comprise at least one of geometrical shape, surface properties, electrical charge properties, or magnetic properties of the obstacles.
4. The device of claim 3 , wherein the asymmetrical surface properties are a result of variation in the binding, affinity or attraction of antibodies, antigens, proteins, or surface molecules.
5. The device of claim 1 , wherein the inner lumen is part of a conduit with at least one inlet and at least one outlet.
6. The device of claim 1 , wherein the separation creates a concentration of particles in one region in the device.
7. The device of claim 1 , wherein the separation depletes the particles in another region in the device.
8. The device of claim 1 , wherein the separation occurs within the device.
9. The device of claim 1 , wherein the separation is for at least one of life science diagnostics, clinical diagnostics, clinical therapeutics, biotechnology analysis, microfluidics, blood components processing, fermentation processes, biotechnology processes or separation of cells.
10. The device of claim 1 , wherein the obstacles comprise multiple groups of obstacles and at least one separation is based on one of the multiple groups.
11. A device for interacting with particles in a particle entrained fluid comprising:
at least one region; and
a group of obstacles in the at least one region, wherein at least a portion of the obstacles in the region each have asymmetrical properties that cause at least some particles from the particle entrained fluid to shift asymmetrically about an individual obstacle resulting in a concentration of particles within at least one portion of the region, further wherein the concentration occurs within the device.
12. The device of claim 11 , wherein the concentration of particles is for at least one of life science diagnostics, clinical diagnostics, clinical therapeutics, biotechnology analysis, microfluidics, blood components processing, fermentation processes, biotechnology processes, or separation of cells.
13. The device of claim 11 , wherein at least a portion of the concentrated particles are treated, processed, or analyzed within the device.
14. The device of claim 11 , wherein the at least one group of obstacles result in a separation or depletion of particles in at least one area of the at least one region.
15. The device of claim 14 , wherein at least a portion of the fluid from which at least some particles have been depleted is processed, treated or analyzed within the device.
16. The device of claim 11 , wherein the asymmetrical properties cause asymmetrical interactions with the obstacles, and further wherein the asymmetrical properties comprise at least one of geometrical shape, surface properties, electrical charge properties, or magnetic properties of the obstacles in the group of obstacles.
17. A device comprising:
a fluid including a plurality of particles entrained in the fluid, and
a plurality of obstacles in a path of the fluid, wherein at least a portion of the individual obstacles each have an asymmetrical property that cause at least some of the particles to shift asymmetrically about an individual obstacle and that cause at least some of the particles to shift symmetrically about an individual obstacle.
18. The device of claim 17 , wherein the asymmetrical properties cause asymmetrical interactions with the obstacles, and further wherein the asymmetrical properties comprise at least one of geometrical shape, surface properties, electrical charge properties, or magnetic properties of the obstacles.
19. The device of claim 17 , wherein at least some individual obstacles cause an asymmetrical particle shift with some particles and a symmetrical particle shift with other particles.
20. The device of claim 17 , wherein the asymmetrical properties of the obstacles are arranged and aligned such that a net separation from the asymmetrical particle shifts is in a different direction than the net separation that the symmetric sifts.
21. The device of claim 17 , wherein the obstacles are arranged and aligned such that a net separation from the asymmetrical particle shifts is in the same direction as the net separation from the symmetric sifts.