IP Library › Granted Patent US 12,342,827
Granted Patent B2
US 12,342,827 · App. 18/597,734 · Granted Jul 1, 2025

Non-toxic pest control compositions and methods and uses thereof

Inventors: Parker Dale (Newport Beach, CA); Parker David Dale (Newport Beach, CA)
Assignee: Neozyme International, Inc.
A01N63/32
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Quick Facts
Patent No.
US 12,342,827
App. No.
18/597,734
Granted
Jul 1, 2025
Kind
B2
Abstract

The present specification discloses pest control compositions, articles of manufacture, containers or kits comprising such compositions, and methods and uses to control a population of invertebrate pests from a mammal, location, plant, structure treated of such pest control compositions.

Claims (23)

1. A method of controlling a population of invertebrate pests, the method comprising:

diluting a pest control composition concentrate by a factor of about 1:1 to about 1:10 with one or more diluents to form a diluted pest control composition, the pest control composition concentrate comprising a treated, fermented yeast supernatant including bio-nutrients, minerals and amino acids, and one or more nonionic surfactants and lacking any active enzymes, activatable pro-enzymes, or any enzymatic activity, wherein after dilution the one or more nonionic surfactants is present in an amount of about 1% to about 15% by weight of the diluted pest control composition, and

applying the diluted pest control composition to a final concentration of about 0.01% to about 0.5% to the population of the invertebrate pests and/or one or more locations where control of the population of the invertebrate pests is desired.

2. The method according to claim 1 , wherein the diluted pest control composition comprises at least 35% by weight of the treated, fermented yeast supernatant.

3. The method according to claim 1 , wherein the diluted pest control composition comprises at most 95% by weight of the treated, fermented yeast supernatant.

4. The method according to claim 1 , wherein a yeast used in a yeast culture to produce a yeast supernatant used as a source to make the treated, fermented yeast supernatant is selected from yeast genera consisting of Brettanomyces, Candida, Cyberlindnera, Cystofilobasidium, Debaryomyces, Dekkera, Geotrichum, Issatchenkia, Kazachstania, Kloeckera, Kluyveromyces, Pediococcus, Penicillium, Pichia, Rhodosporidium, Rhodotorula, Saccharomyces, Schizosaccharomyces, Thrichosporon, Torulaspora, Torulopsis, Yarrowia, Zygosaccharomyces , and Zygotorulaspora.

5. The method according to claim 4 , wherein the yeast culture is a Saccharomyces cerevisiae culture.

6. The method according to claim 1 , wherein the diluted pest control composition comprises from about 3.0% to about 15% by weight of the one or more nonionic surfactants.

7. The method according to claim 6 , wherein the diluted pest control composition comprises from about 5% to about 13% by weight of the one or more nonionic surfactants.

8. The method according to claim 7 , wherein the diluted pest control composition comprises from about 7% to about 11% by weight of the one or more nonionic surfactants.

9. The method according to claim 1 , wherein the one or more nonionic surfactants comprise a polyether nonionic surfactant, a polyhydroxyl nonionic surfactant, and/or a nonionic biosurfactant.

10. The method according to claim 9 , wherein the polyhydroxyl nonionic surfactant comprises a sucrose ester, an ethoxylated sucrose ester, a sorbital ester, an ethoxylated sorbital ester, an alkyl glucoside, an ethoxylated alkyl glucoside, a polyglycerol ester, or an ethoxylated polyglycerol ester.

11. The method according to claim 1 , wherein the one or more nonionic surfactants is an ethoxylated nonyl phenol, an ethoxylated octyl phenol, an ethoxylated ceto-oleyl alcohol, an ethoxylated ceto-stearyl alcohol, an ethoxylated decyl alcohol, an ethoxylated dodecyl alcohol, an ethoxylated tridecyl alcohol, or an ethoxylated castor oil.

12. The method according to claim 1 , wherein the diluted pest control composition further comprises one or more anionic surfactants.

13. The method according to claim 12 , wherein the diluted pest control composition comprises from about 0.5% to about 10% by weight of the one or more anionic surfactants.

14. The method according to claim 13 , wherein the diluted pest control composition comprises from about 1% to about 8% by weight of the one or more anionic surfactants.

15. The method according to claim 14 , wherein the diluted pest control composition comprises from about 2% to about 6% by weight of the one or more anionic surfactants.

16. The method according to claim 1 , wherein the pH of the pest control composition concentrate is at least 3 to at most 4.5.

17. The method according to claim 1 , wherein the invertebrate pests comprise an insect, an arachnid or a nematode.

18. The method according to claim 17 , wherein the invertebrate pests include members in the egg, larval, nymphal, juvenile, pupal, and adult stage of development.

19. The method according to claim 1 , wherein the one or more locations comprises a plant or group of plants or part of a plant, a particular area of land, or a man-made structure.

20. The method according to claim 19 , wherein the particular area of land is a lawn, a garden, a nursery or an agricultural field.

21. The method according to claim 19 , wherein the man-made structure is a commercial building, a residential house, a community facility, a barn, a stable, a shed, a greenhouse or any other physical structure.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 27, 2024
From: DALE, PARKER; DALE, PARKER DAVID
To: NEOZYME INTERNATIONAL, INC.
Reel/Frame 069688/0669 →
Continuity (7)
Continuation 17446961 · Sep 4, 2021
Continuation 16446583 · Jun 19, 2019
Continuation 15243957 · Aug 22, 2016
Continuation In Part 14404917
Provisional Application 62208662 · Aug 22, 2015
Provisional Application 61689077 · May 29, 2012
Related Publication 20240315254A1 · Sep 26, 2024
References Cited (200)
US 3635797A · Battistoni et al. · 1972 [cited by applicant]
US 4052858A · Jeppson et al. · 1977 [cited by applicant]
US 4459213A · Uchida · 1984 [cited by applicant]
US 4541986A · Schwab et al. · 1985 [cited by applicant]
US 4666606A · Heinicke · 1987 [cited by applicant]
US 4758353A · Spence et al. · 1988 [cited by applicant]
US 4804478A · Tamir · 1989 [cited by applicant]
US 5071765A · Wiatr · 1991 [cited by applicant]
US 5075008A · Chigusa et al. · 1991 [cited by applicant]
US 5139945A · Liu · 1992 [cited by applicant]
US 5179003A · Wolf et al. · 1993 [cited by applicant]
US 5227067A · Runyon · 1993 [cited by applicant]
US 5284844A · Lorenz et al. · 1994 [cited by applicant]
US 5308449A · Fuentes et al. · 1994 [cited by applicant]
US 5326477A · Fugua et al. · 1994 [cited by applicant]
US 5369031A · Middleditch et al. · 1994 [cited by applicant]
US 5407577A · Nghiem · 1995 [cited by applicant]
US 5462868A · Britt et al. · 1995 [cited by applicant]
US 5500306A · Hsu et al. · 1996 [cited by applicant]
US 5616479A · Marchal et al. · 1997 [cited by applicant]
US 5654192A · Ducreux et al. · 1997 [cited by applicant]
US 5736209A · Andersen et al. · 1998 [cited by applicant]
US 5807464A · Jobbins et al. · 1998 [cited by applicant]
US 5820758A · Dale et al. · 1998 [cited by applicant]
US 5849566A · Dale et al. · 1998 [cited by applicant]
US 5866376A · Rocha et al. · 1999 [cited by applicant]
US 5879913A · Marchal et al. · 1999 [cited by applicant]
US 5879928A · Dale et al. · 1999 [cited by applicant]
US 5885590A · Hunter et al. · 1999 [cited by applicant]
US 5885950A · Dale et al. · 1999 [cited by applicant]
US 5942552A · Cox · 1999 [cited by applicant]
US 6001218A · Hsu et al. · 1999 [cited by applicant]
US 6699391B2 · Baldridge et al. · 2004 [cited by applicant]
US 6783679B1 · Rozich · 2004 [cited by applicant]
US 6841572B2 · Horst et al. · 2005 [cited by applicant]
US 6884351B1 · Lytal · 2005 [cited by applicant]
US 7165561B2 · Baldridge et al. · 2007 [cited by applicant]
US 7476529B2 · Podella et al. · 2009 [cited by applicant]
US 7645730B2 · Baldridge et al. · 2010 [cited by applicant]
US 7658848B2 · Baldridge et al. · 2010 [cited by applicant]
US 7659237B2 · Baldridge et al. · 2010 [cited by applicant]
US 7759301B2 · Baldridge et al. · 2010 [cited by applicant]
US 7922906B2 · Baldridge et al. · 2011 [cited by applicant]
US 8080186B1 · Pennartz · 2011 [cited by applicant]
US 8188028B2 · Baldridge et al. · 2012 [cited by applicant]
US 8389459B2 · Baldridge et al. · 2013 [cited by applicant]
US 8735338B2 · Baldridge et al. · 2014 [cited by applicant]
US 8778646B1 · Chapman et al. · 2014 [cited by applicant]
US 8821646B1 · Miller · 2014 [cited by applicant]
US 8835152B2 · Podella · 2014 [cited by applicant]
US 8871682B2 · Michalow et al. · 2014 [cited by applicant]
US 8871698B2 · Podella et al. · 2014 [cited by applicant]
US 8894861B2 · Podella et al. · 2014 [cited by applicant]
US 9051535B2 · Goldfeld et al. · 2015 [cited by applicant]
US 9617178B2 · Dale et al. · 2017 [cited by applicant]
US 9713631B2 · Berkes et al. · 2017 [cited by applicant]
US 10334856B2 · Dale et al. · 2019 [cited by applicant]
US 10961275B2 · Bralkowski et al. · 2021 [cited by applicant]
US 20020187220A1 · Luhadiya · 2002 [cited by applicant]
US 20030121868A1 · Barak · 2003 [cited by applicant]
US 20030178162A1 · Raghukumar et al. · 2003 [cited by applicant]
US 20040180411A1 · Podella et al. · 2004 [cited by applicant]
US 20050118106A1 · Schaefer · 2005 [cited by applicant]
US 20050164355A1 · Vlasenko et al. · 2005 [cited by applicant]
US 20050171275A1 · De Jong et al. · 2005 [cited by applicant]
US 20050266036A1 · Awada et al. · 2005 [cited by applicant]
US 20060151387A1 · Yost et al. · 2006 [cited by applicant]
US 20060205042A1 · Aehle et al. · 2006 [cited by applicant]
US 20070029264A1 · Bowe · 2007 [cited by applicant]
US 20070224249A1 · Kelly et al. · 2007 [cited by applicant]
US 20070257127A1 · Iverson · 2007 [cited by applicant]
US 20080138327A1 · Kelly · 2008 [cited by applicant]
US 20080293813A1 · Agvald et al. · 2008 [cited by applicant]
US 20090152196A1 · Podella · 2009 [cited by applicant]
US 20090186761A1 · Arbogast et al. · 2009 [cited by applicant]
US 20100078307A1 · Dale et al. · 2010 [cited by applicant]
US 20100273495A1 · Onggosanusi et al. · 2010 [cited by applicant]
US 20110052514A1 · Justin et al. · 2011 [cited by applicant]
US 20120100236A1 · Asolkar et al. · 2012 [cited by applicant]
US 20120172219A1 · Podella et al. · 2012 [cited by applicant]
US 20130104264A1 · Schoonneveld-Bergmans et al. · 2013 [cited by applicant]
US 20130195826A1 · Alessandri et al. · 2013 [cited by applicant]
US 20130281328A1 · Podella et al. · 2013 [cited by applicant]
US 20130295204A1 · Silberstein · 2013 [cited by applicant]
US 20130313465A1 · Podella et al. · 2013 [cited by applicant]
US 20130344554A1 · Bleyer et al. · 2013 [cited by applicant]
US 20140056853A1 · Marrone et al. · 2014 [cited by applicant]
US 20140128256A1 · Asolkar et al. · 2014 [cited by applicant]
US 20140248373A1 · Michalow et al. · 2014 [cited by applicant]
US 20140290970A1 · Izumida et al. · 2014 [cited by applicant]
US 20150045220A1 · Michalow et al. · 2015 [cited by applicant]
US 20150072917A1 · Baldridge et al. · 2015 [cited by applicant]
US 20150141311A1 · Podella et al. · 2015 [cited by applicant]
US 20150191748A1 · Dale et al. · 2015 [cited by applicant]
US 20150267151A1 · Goldfeld et al. · 2015 [cited by applicant]
US 20160038779A1 · Bown · 2016 [cited by applicant]
US 20160100587A1 · Dywaler-Ekegard et al. · 2016 [cited by applicant]
US 20160298056A1 · Baldridge et al. · 2016 [cited by applicant]
US 20160353746A1 · Dale et al. · 2016 [cited by applicant]
US 20160362834A1 · Dale et al. · 2016 [cited by applicant]
US 20170056455A1 · Berkes et al. · 2017 [cited by applicant]
US 20170156343A1 · Michalow et al. · 2017 [cited by applicant]
US 20180170968A1 · Bralkowski et al. · 2018 [cited by applicant]
US 20190021366A1 · McNeff et al. · 2019 [cited by applicant]
US 20190307130A1 · Dale et al. · 2019 [cited by applicant]
CA 1124459A · 1982 [cited by applicant]
CN 1188679A · 1998 [cited by applicant]
CN 101557249A · 2009 [cited by applicant]
CN 101951686A · 2011 [cited by applicant]
CN 104452385A · 2015 [cited by applicant]
CN 109706093A · 2019 [cited by applicant]
EP 375615A2 · 1990 [cited by applicant]
EP 1721966A1 · 2006 [cited by applicant]
FR 2223453A · 1974 [cited by applicant]
KR 20100088758A1 · 2010 [cited by applicant]
WO 1992011381A1 · 1992 [cited by applicant]
WO 1994012718A · 1994 [cited by applicant]
WO 1996000811A1 · 1996 [cited by applicant]
WO 1997016381A1 · 1997 [cited by applicant]
WO 1997028092A1 · 1997 [cited by applicant]
WO 1998005212A1 · 1998 [cited by applicant]
WO 1998023813A1 · 1998 [cited by applicant]
WO 2000024879A1 · 2000 [cited by applicant]
WO 2001079450A1 · 2001 [cited by applicant]
WO 2002026041A2 · 2002 [cited by applicant]
WO 2003031536A1 · 2003 [cited by applicant]
WO 2003037066A2 · 2003 [cited by applicant]
WO 2005019527A1 · 2005 [cited by applicant]
WO 2005054475A1 · 2005 [cited by applicant]
WO 2005067531A2 · 2005 [cited by applicant]
WO 2005069849A2 · 2005 [cited by applicant]
WO 2006119052A2 · 2006 [cited by applicant]
WO 2008111613A1 · 2008 [cited by applicant]
WO 2010115021A2 · 2010 [cited by applicant]
WO 2010148535A1 · 2010 [cited by applicant]
WO 2011016008A1 · 2011 [cited by applicant]
WO 2012040908A1 · 2012 [cited by applicant]
WO 2012051328A2 · 2012 [cited by applicant]
WO 2013180756A1 · 2013 [cited by applicant]
WO 2017035099A1 · 2017 [cited by applicant]
WO 2017035100A1 · 2017 [cited by applicant]
WO 2017035101A1 · 2017 [cited by applicant]
WO 2003035972A1 · 2023 [cited by applicant]
Chaichi, et al., Surfactant Application on Yield and Irrigation Water Use Efficiency in Corn under Limited Irrigation, Crop Sci. 55(1): 386 (2015). [cited by applicant]
Desai, et al., Microbial Production of Surfactants and Their Commercial Potential, Microbiol. Mol. Biol. Rev. 61(1): 47-64 (1997). [cited by applicant]
Frolund, et al., Enzymatic Activity in the Activated-Sludge Floc Matrix, Appl. Microbiol. Biotechnol. 43(3): 755-561 (1995). Abstract Only. [cited by applicant]
Goel, et al., Enzyme Activities under Anaerobic and Aerobic Conditions in Activated Sludge Sequencing Batch Reactor, Water Research 32(7): 2081-2088 (1998). [cited by applicant]
Ito, et al., Sophorolipids from Torulopsis bombicola: Possible Relation to Alkane Update, Appl. Environ, Micobiol. 43 (6): 1278-1283 (1982). [cited by applicant]
Kastner, et al., Formation of Bound Residues during Microbial Degradation of [14C]Anthracene in Soil, Appl. Environ. Microbiol. 65(5): 1834-1842 (1999). [cited by applicant]
Sensient Flavors LLC, Tastone 154, Technical Information (2010). [cited by applicant]
Sukumaran, et al., Microbial Celluloses—Production, Applications, and Challenges, J. Sci. Indus. Res. 64: 832-844 (2005). [cited by applicant]
Witek-Krowiak, et al., Ultrafiltrative Separation of Rhamnolipid from Culture Medium, World J. Microbiol. Biotechnol. 27: 1961-1964 (2011). [cited by applicant]
Xu, et al., Research Review of Wastewater Treatment Technology with Hydrolytic Enzymes, J. Chongqing Univ. Sci. Technol. 12(6): 156-161 (2010). [cited by applicant]
Xu, et al., Biosurfactants for Microbubble Preparation and Application, Int. J. Mol. Sci. 12: 462-475 (2011). [cited by applicant]
EPO, Extended Search Report for European Patent Application Serial No. EP13796699.0, pp. 12 (Jul. 12, 2016). [cited by applicant]
EPO, Extended Search Report for European Patent Application Serial No. EP16839956.6, pp. 12 (Jan. 14, 2019). [cited by applicant]
EPO, Extended Search Report for European Patent Application Serial No. EP19160826.4, pp. 5 (Apr. 3, 2019). [cited by applicant]
EPO, Extended Search Report for European Patent Application Serial No. EP16839957.4, pp. 11 (Apr. 17, 2019). [cited by applicant]
EPO, Extended Search Report for European Patent Application Serial No. EP16839958.2, pp. 12 (Sep. 30, 2019). [cited by applicant]
WIPO, PCT Form ISA210, International Search Report for International Patent Application Serial No. PCT/US2013/000140, pp. 2 (Jul. 22, 2013). [cited by applicant]
WIPO, PCT Form ISA237, Written Opinion for International Patent Application Serial No. PCT/US2013/000140, pp. 11 (Jul. 22, 2013). [cited by applicant]
WIPO, PCT Form IB373, International Preliminary Report on Patentability for International Patent Application Serial No. PCT/US2013/000140, pp. 12 (Dec. 2, 2014). [cited by applicant]
WIPO, PCT Form ISA210, International Search Report for International Patent Application Serial No. PCT/US2016/048092, pp. 10 (Nov. 15, 2016). [cited by applicant]
WIPO, PCT Form ISA237, Written Opinion for International Patent Application Serial No. PCT/US2016/048092. pp. 5 (Nov. 15, 2016). [cited by applicant]
WIPO, PCT Form IB373, International Preliminary Report on Patentability for International Patent Application Serial No. PCT/US2016/048092, pp. 6 (Mar. 8, 2018). [cited by applicant]
WIPO, PCT Form ISA210, International Search Report for International Patent Application Serial No. PCT/US2016/048093, pp. 8 (Oct. 24, 2016). [cited by applicant]
WIPO, PCT Form ISA237, Written Opinion for International Patent Application Serial No. PCT/US2016/0048093, pp. 4 (Oct. 24, 2016). [cited by applicant]
WIPO, PCT Form IB373, International Preliminary Report on Patentability for International Patent Application Serial No. PCT/US2016/048093, pp. 5 (Mar. 8, 2018). [cited by applicant]
WIPO, PCT Form ISA210, International Search Report for International Patent Application Serial No. PCT/US2016/048094, pp. 7 (Nov. 4, 2016). [cited by applicant]
WIPO, PCT Form ISA237, Written Opinion for International Patent Application Serial No. PCT/US2016/048094, pp. 4 (Nov. 4, 2016). [cited by applicant]
WIPO, PCT Form IB373, International Preliminary Report on Patentability for International Patent Application Serial No. PCT/US2016/048094, pp. 5 (Mar. 8, 2018). [cited by applicant]
Haruhiko, Recent Yeast Taxonomy, J. Brewing Society of Japan, 80(8): 519-529 (1985). [cited by applicant]
Hirata, et al., Novel Characteristics of Sophorolipids, Yeast Glycolipid Biosurfactants, as Biodegradable Low-Foaming Surfactants, J. Biosci. Bioengin. 108(2): 142-146 (2009). [cited by applicant]
Luft, et al., Molecular Dynamics Simulation of the Oil Sequestration Properties of a Nonionic Rhamnolipid, J. Phy. Chem. B 122: 3944-3952 (2018). [cited by applicant]
Tucker, et al., Surfactant/Biosurfactant Mixing: Adsorption of Saponin/Nonionic Surfactant Mixtures at the Air-Water Interface, J. Colloid Interface Sci. (2020), 574: 385-392. [cited by applicant]
WIPO, PCT Form ISA210, International Search Report for International Patent Application Serial No. PCT/ US2021/029238, pp. 3 (Jul. 21, 2021). [cited by applicant]
WIPO, PCT Form ISA237, Written Opinion for International Patent Application Serial No. PCT/US2021/029238, pp. 7 (Jul. 21, 2021). [cited by applicant]
WIPO, PCT Form IB373, International Preliminary Report on Patentability for International Patent Application Serial No. PCT/US2021/029238, pp. 9 (Nov. 10, 2022). [cited by applicant]
WIPO, PCT Form ISA210, International Search Report for International Patent Application Serial No. PCT/US2021/029240, pp. 4 (Jul. 21, 2021). [cited by applicant]
WIPO, PCT Form ISA237, Written Opinion for International Patent Application Serial No. PCT/US2021/029240, pp. 8 (Jul. 21, 2021). [cited by applicant]
WIPO, PCT Form IB373, International Preliminary Report on Patentability for International Patent Application Serial No. PCT/US2021/029240, pp. 10 (Nov. 10, 2022). [cited by applicant]
CNIPA, Second Office Action for Chinese Patent Application Serial No. 201680061816.2, pp. 9 (Mar. 15, 2021). [cited by applicant]
JPO, Office Action for Japanese Patent Application Serial No. 2018-528937, pp. 2 (Jul. 13, 2021). [cited by applicant]
JPO, Office Action for Japanese Patent Application Serial No. 2018-528938, pp. 4 (Aug. 17, 2021). [cited by applicant]
U.S. Appl. No. 15/243,957, filed Aug. 22, 2016, US20160360758, U.S. Pat. No. 10,334,856. [cited by applicant]
U.S. Appl. No. 16/446,583, filed Jun. 19, 2019, US 20190307130, U.S. Pat. No. 11,116,224. [cited by applicant]
U.S. Appl. No. 17/446,961, filed Sep. 4, 2021, US 2021/0392903, U.S. Pat. No. 11,930,823. [cited by applicant]
U.S. Appl. No. 14/404,917, filed May 24, 2013, US 2015/0191748, U.S. Pat. No. 9,617,178. [cited by applicant]
U.S. Appl. No. 15/243,958, filed Aug. 22, 2016, US 2016/0362834, U.S. Pat. No. 10,557,234. [cited by applicant]
U.S. Appl. No. 15/243,961, filed Aug. 22, 2016, US 2016/0353746, U.S. Pat. No. 10,681,914. [cited by applicant]
U.S. Appl. No. 15/444,093, filed Feb. 27, 2017, US 2017/0166467, U.S. Pat. No. 10,683,222. [cited by applicant]
U.S. Appl. No. 16/729,236, filed Dec. 27, 2019, US 2020/0131701, U.S. Pat. No. 11,773,535. [cited by applicant]
U.S. Appl. No. 16/729,240, filed Dec. 27, 2019, US 2020/0138037, U.S. Pat. No. 11,771,091. [cited by applicant]
U.S. Appl. No. 16/729,243, filed Dec. 27, 2019, US 2020/0140304, U.S. Pat. No. 11,772,996. [cited by applicant]
U.S. Appl. No. 17/240,919, filed Apr. 26, 2021, US 2021/0329913, U.S. Pat. No. 12,010,992. [cited by applicant]
U.S. Appl. No. 17/240,925, filed Apr. 26, 2021, US 2021/0331017. [cited by applicant]
U.S. Appl. No. 18/460,056, filed Sep. 1, 2023, US 2023/0416127. [cited by applicant]
U.S. Appl. No. 18/460,092, filed Sep. 1, 2023, US 2024/0003084. [cited by applicant]
U.S. Appl. No. 18/460,129, filed Sep. 1, 2023, US 2023/0404083. [cited by applicant]
U.S. Appl. No. 18/745,572, filed Jun. 17, 2024. [cited by applicant]