Patent
Exemplary embodiments of the present disclosure may include an isolated mycelium of Trametes versicolor designated as strain M2-101-03. Exemplary embodiments of the present disclosure may also include a method for propagating a Trametes versicolor mycelium, the method including culturing a mother culture, culturing a production culture, the production culture being a sub-culture of the mother culture, creating a liquid master inoculum using the production culture, diluting the liquid master inoculum to create a diluted liquid master inoculum, creating a biomass product using the diluted liquid master inoculum, slicing the biomass product for dehydration to create a sliced biomass product, dehydrating the sliced biomass product to create a dehydrated sliced biomass product, milling the dehydrated sliced biomass product to select a designated particle size of the dehydrated sliced biomass product, and screening the designated particle size of the dehydrated sliced biomass product.
1 . An isolated mycelium of Trametes versicolor designated as strain M2-101-03 as deposited at the ATCC Patent Depository under the Budapest Treaty (10801 University Boulevard, Manassas, VA 20110), on Jul. 28, 2023, with the official deposit date of Jul. 28, 2023, and the official Patent Deposit Designation No. PTA-127611, wherein the isolated mycelium is purified.
2 . The isolated mycelium of claim 1 , characterized by a phenotype having a distributed mass of fruitbodies on top and sides of a substrate.
3 . The isolated mycelium of claim 1 , characterized by a phenotype having fruiting initiation between fifteen to twenty-four days after inoculation.
4 . The isolated mycelium of claim 1 , comprising amylase enzymes, the amylase enzymes breaking down starches of oat agar to sugars to enable growth of the isolated mycelium on the oat agar.
5 . The isolated mycelium of claim 1 , characterized by a phenotype having a beta-glucan content of 20% to 35%.
6 . The isolated mycelium of claim 1 , characterized by a phenotype having a beta-glucan content of 25% to 35%.
7 . The isolated mycelium of claim 1 , characterized by a phenotype having a beta-glucan content of 20% to 30%.
8 . The isolated mycelium of claim 1 , characterized by a phenotype having a beta-glucan content of 25% to 30%.
9 . The isolated mycelium of claim 1 , characterized by a phenotype having a beta-glucan content of 20% to 25%.
10 . The isolated mycelium of claim 1 , characterized by a phenotype having a beta-glucan content of 25%.
11 . The isolated mycelium of claim 1 , wherein the isolated mycelium is in a powder form.
12 . The isolated mycelium of claim 1 , wherein the isolated mycelium is in a capsule form.
13 . The isolated mycelium of claim 1 , wherein the isolated mycelium is in a hot beverage form.
14 . The isolated mycelium of claim 1 , further comprising various concentrations of polysaccharide peptides.
15 . A method for propagating a Trametes versicolor mycelium, the method comprising:
culturing a mother culture;
culturing a production culture, the production culture being a sub-culture of the mother culture;
creating a liquid master inoculum using the production culture;
diluting the liquid master inoculum to create a diluted liquid master inoculum;
creating a biomass product using the diluted liquid master inoculum;
slicing the biomass product for dehydration to create a sliced biomass product;
dehydrating the sliced biomass product to create a dehydrated sliced biomass product;
milling the dehydrated sliced biomass product to select a designated particle size of the dehydrated sliced biomass product;
screening the designated particle size of the dehydrated sliced biomass product; and
culturing the production culture onto four nutrient plates.
16 . The method for propagating a Trametes versicolor mycelium of claim 15 , the method further comprising:
culturing the mother culture on a mixture comprising approximately 10 grams of dextrose, approximately 2.5 grams of potato starch, approximately 10 grams of agar, approximately 5 grams of cauliflower, approximately 5 grams of nutritional yeast and approximately 1 liter of water.
17 . The method for propagating a Trametes versicolor mycelium of claim 15 , the method further comprising:
creating the liquid master inoculum using the production culture comprising approximately one pound of hydrated hulled oats hydrated to an approximately 50% to 60% moisture content in a water bath at approximately 190 to 195 degrees Fahrenheit for approximately 30 minutes to 60 minutes.
18 . The method for propagating a Trametes versicolor mycelium of claim 15 , the method further comprising:
screening the designated particle size of the dehydrated sliced biomass product to approximately 60 mesh.
19 . An isolated mycelium of Trametes versicolor designated as strain M2-101-03 as deposited at the ATCC Patent Depository under the Budapest Treaty (10801 University Boulevard, Manassas, VA 20110), on Jul. 28, 2023, with the official deposit date of Jul. 28, 2023, and the official Patent Deposit Designation No. PTA-127611, further comprising various concentrations of polysaccharide peptides.