Solid medium for
The present disclosure relates to the technical field of culture materials for fungi and provides a solid medium for Coriolus versicolor , and a preparation method and use. Bran is used as a main component in the solid medium, and has advantages in economy and environmental protection, and provides high Coriolus versicolor growth rate and strong contamination resistance. The solid medium avoids the tendency to contamination of current media during an experimental process, and reduces culture cost. The solid medium provides higher biomass and stronger contamination resistance than a potato dextrose agar (PDA) medium.
1. A solid medium for Coriolus versicolor, comprising the following raw materials: bran, xylose, a trace element solution, agar, and water; wherein the bran and xylose have a mass ratio of 8:3 to 1:1;
wherein trace elements in the trace element solution comprise Mg, Mn, Na, Fe, Co, Ca, Zn, Cu, K, B, and Mo, and the trace elements in the trace element solution are provided by MgSO 4 , MnSO 4 , NaCl, FeSO 4 , CoSO 4 , CaCl 2 , ZnSO 4 , CuSO 4 , KAI(SO 4 ) 2 , H 3 BO 3 , and NaMoO 4 .
2. The solid medium according to claim 1 , wherein MgSO 4 is provided in a form of MgSO 4 ·7H 2 O, FeSO 4 is provided in a form of FeSO 4 ·7H 2 O, and CuSO 4 is provided in a form of CuSO 4 ·5H 2 O.
3. The solid medium according to claim 2 , wherein the trace element solution comprises 3.0 g/L of MgSO 4 ·7H 2 O, 0.5 g/L of MnSO 4 , 1.0 g/L of NaCl, 0.1 g/L of FeSO 4 ·7H 2 O, 0.1 g/L of CoSO 4 , 0.082 g/L of CaCl 2 , 0.1 g/L of ZnSO 4 , 0.01 g/L of CuSO 4 ·5H 2 O, 0.01 g/L of KAI(SO 4 ) 2 , 0.01 g/L of H 3 BO 3 , and 0.01 g/L of NaMoO 4 .
4. The solid medium according to claim 1 , wherein each liter of the solid medium comprises 1.36 mL to 2 mL of the trace element solution.
5. The solid medium according to claim 3 , wherein each liter of the solid medium comprises 1.36 mL to 2 mL of the trace element solution.
6. A preparation method of the solid medium according to claim 1 , comprising the following steps when preparing each liter of the solid medium: mixing the bran with 500 mL of boiled water, boiling for 25 min, and filtering to collect a bran extract; and
mixing the bran extract with xylose, the agar, the trace element solution, and water, boiling, diluting to 1 liter with water, and sterilizing and cooling to obtain the solid medium.
7. The preparation method according to claim 6 , wherein the filtering is conducted by using a 9-layer gauze.
8. A method for culturing Coriolus versicolor, comprising the following steps: inoculating a strain of the Coriolus versicolor on the solid medium according to claim 1 , sealing, and culturing at 28° C.