IP Library › Granted Patent US 12,258,600
Granted Patent B2
US 12,258,600 · App. 18/693,613 · Granted Mar 25, 2025

Method for the fermentative production of L-lysine using

Inventors: Silke Jerrentrup (Bielefeld, DE); Brigitte Bathe (Salzkotten, DE); Kornelia Voß (Antwerp, BE); Frank Jankowitsch (Sassenberg, DE)
Assignee: Evonik Operations GmbH
C12P13/08C07K14/34C12N9/1217C12Y207/02004
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Quick Facts
Patent No.
US 12,258,600
App. No.
18/693,613
Granted
Mar 25, 2025
Kind
B2
Abstract

C. glutamicum strains express modified gluconate repressor proteins GntR1 and GntR2. The C. glutamicum strains have an increased ability to produce L-lysine compared with an ability of a wildtype strain. The gluconate repressor protein GntR1 includes an amino acid sequence of SEQ ID NO: 9, where the amino acid Arg in position 102 is replaced by the amino acid Glu. The gluconate repressor protein GntR2 includes SEQ ID NO: 10, and its activity is attenuated compared to the activity of the GntR2 repressor protein of the wild-type strain. A method is developed for the fermentative production of L-lysine using such C. glutamicum strains.

Claims (14)

1. A Corynebacterium glutamicum strain having an increased ability to produce L-lysine compared with an ability of a wildtype strain, comprising:

a gene coding for a mutant GntR1 protein comprising the amino acid sequence of SEQ ID NO: 9 except for a R102E substitution and

an inactivated gluconate repressor protein GntR2 gene.

2. The Corynebacterium glutamicum strain of claim 1 , further comprising at least one copy of a gene lysC coding for a feedback resistant aspartokinase polypeptide variant.

3. The Corynebacterium glutamicum strain of claim 2 , wherein an amino acid sequence of the feedback resistant aspartokinase polypeptide variant comprises SEQ ID NO: 11.

4. The Corynebacterium glutamicum strain of claim 1 , comprising in its chromosome a polynucleotide encoding an amino acid sequence of a polypeptide having gluconate repressor activity comprising the amino acid sequence according to SEQ ID NO:5.

5. The Corynebacterium glutamicum strain of claim 1 , wherein a gene gntR2 coding for the gluconate repressor protein GntR2 is deleted.

6. A method for a fermentative production of an L-lysine, comprising:

cultivating the Corynebacterium glutamicum strain of claim 1 , and

accumulating L-lysine in a medium to form an L-lysine containing fermentation broth.

7. The method of claim 6 , further comprising:

manufacturing an L-lysine containing product from said fermentation broth.

8. The method of claim 7 , further comprising:

isolating L-lysine from the L-lysine containing fermentation broth.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 27, 2024
From: JERRENTRUP, SILKE; BATHE, BRIGITTE; VOß, KORNELIA; JANKOWITSCH, FRANK
To: EVONIK OPERATIONS GMBH
Reel/Frame 066916/0169 →
Priority Claims (1)
EP 21197722 · Sep 20, 2021 · regional
Continuity (1)
Related Publication 20240271169A1 · Aug 15, 2024
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