Method for the fermentative production of L-lysine
A bacterium of the species Corynebacterium glutamicum has the ability to excrete L-lysine, and contains in its chromosome a polynucleotide encoding a mutated polypeptide having the assumed function of an acyltransferase, hydrolase, alpha/beta hydrolase or of a pimeloyl-ACP methyl ester esterase. Also, a method is used for producing L-lysine using such bacterium.
1. A bacterium of the species Corynebacterium glutamicum , comprising:
an ability to excrete L-lysine, and
a polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, except the serine at the position corresponding to position 225 of the amino acid sequence of SEQ ID NO: 2 is substituted with a different amino acid, and wherein the polynucleotide is contained in the chromosome of the bacterium.
2. The bacterium of claim 1 , wherein the serine at the position corresponding to position 225 of the amino acid sequence of SEQ ID NO: 2 is substituted with cysteine.
3. The bacterium of claim 2 , wherein the polynucleotide comprises the nucleotide sequence of nucleotides 328 to 1320 of SEQ ID NO: 1 with guanine at the position corresponding to position 1001 of SEQ ID NO: 1.
4. The bacterium of claim 2 , wherein the polynucleotide comprises the nucleotide sequence of nucleotides 328 to 1323 of SEQ ID NO: 1 with guanine at the position corresponding to position 1001 of SEQ ID NO: 1.
5. The bacterium of claim 1 , comprising at least one copy of a polynucleotide comprising a nucleotide sequence encoding a feedback resistant aspartokinase polypeptide, wherein the feedback resistant aspartokinase polypeptide comprises the amino acid sequence of SEQ ID NO: 6.
6. The bacterium of claim 1 , comprising at least one copy of a polynucleotide comprising a nucleotide sequence encoding for a feedback resistant aspartokinase polypeptide comprising the amino acid sequence of SEQ ID NO: 6, except the threonine at the position corresponding to position 311 of the amino acid sequence of SEQ ID NO: 6 is substituted with isoleucine.
7. A method for fermentative production of L-lysine, comprising:
a) cultivating the bacterium of claim 1 in a suitable medium under suitable conditions for the production of L-lysine, and
b) accumulating L-lysine in the suitable medium to form an L-lysine containing fermentation broth.
8. The method of claim 7 , further comprising:
purifying L-lysine.
9. The method of claim 8 , wherein L-lysine is purified by at least one process selected from the group consisting of treatment with activated carbon, ionic exchange, and crystallization.