Granted Patent B2

US 12,274,754 · App. 18/821,707 · Granted Apr 15, 2025

Inventors: Xiaojun Han (San Diego, CA); Suvi Tuula Marjukka Orr (San Diego, CA); Kevin Duane Bunker (Escondido, CA); Peter Qinhua Huang (San Diego, CA); Kimberlee Fischer (San Diego, CA)

Assignee: Immunome, Inc.

A61K47/6803A61K31/4745A61K47/55A61K47/6851C07K16/18A61K2039/505

Abstract

Claims (44)

1. An immunoconjugate represented by:

wherein Ab is an antibody or an antigen-binding fragment thereof, and n is an integer from 1 to 10.

2. The immunoconjugate of claim 1 , wherein Ab specifically binds human receptor tyrosine kinase like orphan receptor 1 (ROR1).

3. The immunoconjugate of claim 1 , wherein Ab comprises:

a) a variable heavy chain region (VH) comprising:

(i) a VHCDR1 comprising the amino acid sequence of SEQ ID NO: 1;

(ii) a VHCDR2 comprising the amino acid sequence of SEQ ID NO: 2; and

(iii) a VHCDR3 comprising the amino acid sequence of SEQ ID NO: 3; and

b) a variable light chain region (VL) comprising:

(i) a VLCDR1 comprising the amino acid sequence of SEQ ID NO: 8;

(ii) a VLCDR2 comprising the amino acid sequence AAS; and

(iii) a VLCDR3 comprising the amino acid sequence of SEQ ID NO: 10.

4. The immunoconjugate of claim 1 , wherein Ab comprises:

a) a variable heavy chain region (VH) comprising at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 5 and maintains the sequences of VHCDR1 as set forth in SEQ ID NO: 1; VHCDR2 as set forth in SEQ ID NO: 2; and VHCDR3 as set forth in SEQ ID NO: 3; and

b) a variable light chain region (VL) comprising at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 12 and maintains the sequences of VLCDR1 as set forth in SEQ ID NO: 8; a VLCDR2 of AAS; and VLCDR3 as set forth in SEQ ID NO: 10.

5. The immunoconjugate of claim 1 , wherein Ab comprises:

a) a variable heavy chain region (VH) comprising:

(i) a VHCDR1 comprising the amino acid sequence of SEQ ID NO: 15;

(ii) a VHCDR2 comprising the amino acid sequence of SEQ ID NO: 16; and

(iii) a VHCDR3 comprising the amino acid sequence of SEQ ID NO: 17; and

b) a variable light chain region (VL) comprising:

(i) a VLCDR1 comprising the amino acid sequence of SEQ ID NO: 22;

(ii) a VLCDR2 comprising the amino acid sequence DAY; and

(iii) a VLCDR3 comprising the amino acid sequence of SEQ ID NO: 24.

6. The immunoconjugate of claim 1 , wherein Ab comprises:

a) a variable heavy chain region (VH) comprising at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 19 and maintains the sequences of VHCDR1 as set forth in SEQ ID NO: 15; VHCDR2 as set forth in SEQ ID NO: 16; and VHCDR3 as set forth in SEQ ID NO: 17; and

b) a variable light chain region (VL) comprising at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 26 and maintains the sequences of VLCDR1 as set forth in SEQ ID NO: 22; a VLCDR2 of DAY; and VLCDR3 as set forth in SEQ ID NO: 24.

7. The immunoconjugate of claim 1 , wherein Ab comprises:

a) a variable heavy chain region (VH) comprising:

(i) a VHCDR1 comprising the amino acid sequence of SEQ ID NO: 29;

(ii) a VHCDR2 comprising the amino acid sequence of SEQ ID NO: 30; and

(iii) a VHCDR3 comprising the amino acid sequence of SEQ ID NO: 31; and

b) a variable light chain region (VL) comprising:

(i) a VLCDR1 comprising the amino acid sequence of SEQ ID NO: 36;

(ii) a VLCDR2 comprising the amino acid sequence DAS; and

(iii) a VLCDR3 comprising the amino acid sequence of SEQ ID NO: 38.

8. The immunoconjugate of claim 1 , wherein Ab comprises:

a) a variable heavy chain region (VH) comprising at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 33 and maintains the sequences of VHCDR1 as set forth in SEQ ID NO: 29; VHCDR2 as set forth in SEQ ID NO: 30; and VHCDR3 as set forth in SEQ ID NO: 31; and

b) a variable light chain region (VL) comprising at least 80% sequence identity to the amino acid sequence of SEQ ID NO: 40 and maintains the sequences of VLCDR1 as set forth in SEQ ID NO: 36; a VLCDR2 of DAS; and VLCDR3 as set forth in SEQ ID NO: 38.

9. A pharmaceutical composition comprising the immunoconjugate of claim 1 , and a pharmaceutically acceptable excipient.

10. A conjugate represented by:

or a pharmaceutically acceptable salt thereof.

11. A method of treating a cancer or a tumor comprising administering an effective amount of the immunoconjugate of claim 1 to a subject having the cancer or the tumor.

12. The method of claim 11 , wherein the cancer or the tumor is selected from lung cancer, urothelial cancer, colorectal cancer, prostate cancer, ovarian cancer, pancreatic cancer, breast cancer, bladder cancer, gastric cancer, gastrointestinal stromal tumor, uterine cervix cancer, esophageal cancer, squamous cell carcinoma, peritoneal cancer, liver cancer, hepatocellular cancer, colon cancer, rectal cancer, colorectal cancer, endometrial cancer, uterine cancer, salivary gland cancer, kidney cancer, vulval cancer, thyroid cancer, penis cancer, leukemia, malignant lymphoma, plasmacytoma, myeloma, or sarcoma.

Assignments (4)

Continuity (6)

References Cited (202)