Aerial mycelia and methods of making same
This application relates generally to aerial mycelium and methods of making aerial mycelium suitable for use as a food or textile product or ingredient. The aerial mycelium can be grown using a growth matrix provided into a growth environment and introducing aqueous mist into the growth environment. The aqueous mist has a mean mist deposition rate to allow for aerial mycelial growth from the growth matrix.
1 . A method of making an aerial mycelium, comprising:
incubating a growth matrix in a growth environment for an incubation time period, wherein the growth matrix comprises a substrate and a fungus;
introducing aqueous mist into the growth environment throughout the incubation time period or a portion thereof; and
producing extra-particle aerial mycelial growth from the growth matrix;
wherein introducing comprises introducing the aqueous mist into the growth environment resulting in a mean mist deposition rate of at most about 0.45 microliter/cm 2 /hour.
2 . The method of claim 1 , wherein introducing comprises introducing the aqueous mist into the growth environment resulting in an instantaneous mist deposition rate and the mean mist deposition rate, wherein the ratio of the instantaneous mist deposition rate to the mean mist deposition rate is at most about 20 to about 1.
3 . The method of claim 2 , wherein the ratio of the instantaneous mist deposition rate to the mean mist deposition rate is at most about 10 to about 1.
4 . The method of claim 2 , wherein the ratio of the instantaneous mist deposition rate to the mean mist deposition rate is at most about 5 to about 1.
5 . The method of claim 1 , wherein:
the growth environment comprises a misting apparatus; and
introducing comprises introducing the aqueous mist into the growth environment via the misting apparatus, resulting in the mean mist deposition rate;
wherein the misting apparatus is operated at a duty cycle, and wherein the duty cycle is at least about 5%.
6 . The method of claim 5 , wherein the duty cycle is at least about 10%.
7 . The method of claim 5 , wherein the duty cycle is at least about 20%.
8 . The method of claim 1 , wherein introducing further comprises introducing the aqueous mist into the growth environment resulting in an instantaneous mist deposition rate of at most about 2 microliter/cm 2 /hour.
9 . The method of claim 1 , wherein the mean mist deposition rate is at least about 0.01 microliter/cm 2 /hour.
10 . The method of claim 1 , wherein the mean mist deposition rate is at least about 0.03 microliter/cm 2 /hour.
11 . The method of claim 1 , wherein the mean mist deposition rate is at least about 0.05 microliter/cm 2 /hour.
12 . The method of claim 1 , wherein said growth environment comprises a growth atmosphere, said growth atmosphere having a carbon dioxide content, wherein said carbon dioxide content modulates with fungal respiration over the course of the incubation time period.
13 . The method of claim 1 , wherein said growth environment comprises a growth atmosphere, said growth atmosphere having a carbon dioxide content, wherein said carbon dioxide content is not a preselected carbon dioxide content.
14 . The method of claim 1 , wherein said growth environment comprises a growth atmosphere, said growth atmosphere having a relative humidity, an oxygen (O 2 ) content, and a carbon dioxide content, wherein the carbon dioxide content is at least about 0.02% (v/v) and less than about 8% (v/v).
15 . The method of claim 14 , wherein the CO 2 content is within a range of about 0.2% (v/v) to about 7% (v/v).
16 . The method of claim 14 , wherein the CO 2 content is within a range of about 3% (v/v) to about 7% (v/v).
17 . The method of claim 1 , wherein the aqueous mist comprises a conductivity, wherein the conductivity is no greater than about 50 microsiemens/cm.