IP Library Granted Patent US 12,416,552
Granted Patent B2
US 12,416,552 · App. 18/921,957 · Granted Sep 16, 2025

Methods and devices for manipulating biological samples

Inventors: Yuji Ishitsuka (San Diego, CA); Jaekyung Koh (San Diego, CA); Robert James Stover (San Diego, CA); Pavel Shekhtmeyster (San Diego, CA); Sandor Kovacs (Middletown, DE); Hu Cang (San Diego, CA); Valeria Rascon (San Diego, CA)
Assignee: Singular Genomics Systems, Inc.
G01N1/30G01N1/286G01N1/312G01N2001/288G01N2001/305G01N2001/315
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Quick Facts
Patent No.
US 12,416,552
App. No.
18/921,957
Granted
Sep 16, 2025
Kind
B2
Abstract

Disclosed herein, inter alia, are devices and methods for efficient transfer and analyses of cellular material, tissue samples, such as tissue sections, using carrier substrates and devices.

Claims (16)

1. A system comprising:

a glass solid support;

a receiving array removably attached to the glass solid support, comprising a plurality of receptacles each forming an internal cavity, wherein one or more receptacles comprise a cell or tissue sample; and

a piston array comprising a base and a plurality of spring actuated pistons extending from the base and wherein the pistons are sized and shaped to fit within the internal cavity of the receptacles.

2. The system of claim 1 , wherein the glass solid support further comprises a resist.

3. The system of claim 1 , wherein the glass solid support further comprises polylysine, poly(2-dimethylaminoethyl methacrylate) (PDMAEMA), chitosan, poly(amidoamine) (PAMAM), polyvinylamine (PVAm), or poly(allylamine hydrochloride) (PAH).

4. The system of claim 1 , wherein the tissue sample comprises breast tissue, lung tissue, colon tissue, lymph tissue, kidney tissue, bone tissue, tonsil tissue, or brain tissue.

5. The system of claim 4 , wherein a thickness of the tissue is about 1 μm to about 20 μm.

6. The system of claim 4 , wherein a thickness of the tissue is about 3 μm to about 10 μm.

7. The system of claim 4 , wherein a thickness of the tissue is about 5 μm to about 7 μm.

8. The system of claim 1 , wherein the tissue sample is attached to a carrier substrate forming a sample-carrier construct, wherein said carrier substrate comprises agarose, amylose, amylopectin, alginate, gelatin, cellulose, polyolefin, polyethylene glycol, polyvinyl alcohol, and/or acrylate polymers and copolymers thereof.

9. The system of claim 8 , wherein the pistons comprise a piston head, wherein the piston head is configured to contact the carrier substrate.

10. The system of claim 9 , wherein a diameter of the piston head is less than a diameter of the piston.

11. The system of claim 1 , wherein the tissue sample is attached to a carrier substrate forming a sample-carrier construct, wherein said carrier substrate comprises agarose, agar, amylopectin, polyvinyl alcohol, Gellan gum, or alginate.

12. The system of claim 1 , wherein each receptacle is cylindrical, square, or rectangular.

13. The system of claim 1 , wherein the receiving array comprises an identifying feature at each receptacle, wherein the identifying feature uniquely identifies the receptacle.

Assignments (2)
SECURITY INTEREST Recorded Mar 7, 2025
From: SINGULAR GENOMICS SYSTEMS, INC.
To: FIRST-CITIZENS BANK & TRUST COMPANY
Reel/Frame 070440/0465 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 6, 2024
From: ISHITSUKA, YUJI; KOH, JAEKYUNG; SHEKHTMEYSTER, PAVEL; STOVER, ROBERT JAMES; KOVACS, SANDOR; CANG, HU; RASCON, VALERIA
To: SINGULAR GENOMICS SYSTEMS, INC.
Reel/Frame 069511/0497 →
Continuity (5)
Continuation PCTUS2024013802 · Jan 31, 2024
Provisional Application 63617636 · Jan 4, 2024
Provisional Application 63513834 · Jul 14, 2023
Provisional Application 63482727 · Feb 1, 2023
Related Publication 20250044197A1 · Feb 6, 2025
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