IP Library › Granted Patent US 12,297,488
Granted Patent B2
US 12,297,488 · App. 18/972,148 · Granted May 13, 2025

Spatially encoded biological assays

Inventor: Mark S. Chee (San Diego, CA)
Assignee: Prognosys Biosciences, Inc.
C12Q1/6837B01L3/502715C12Q1/68C12Q1/6804C12Q1/6809C12Q1/6834C12Q1/6841C12Q1/6869C12Q1/6874C12Q1/6876C40B30/04C40B60/04G01N33/5308G01N33/543G01N33/54366G01N33/6845G01N33/6848C12Q1/6858C12Q2600/156C12Q2600/158G01N2458/10G01N2458/40
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12,297,488
App. No.
18/972,148
Filed
Dec 6, 2024
Granted
May 13, 2025
Kind
B2
Art Unit
1683
USPC
506/16
Abstract

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

Claims (41)

1. A method for determining a presence of a nucleic acid at a location in a tissue section comprising:

(a) contacting the tissue section with a substrate comprising a plurality of features, wherein a feature of the plurality of features comprises a capture agent, wherein the capture agent comprises:

(i) a first sequence that binds the nucleic acid, and

(ii) a second sequence comprising a coding tag, wherein a sequence of the coding tag corresponds to a location of the capture agent on the substrate;

(b) determining the sequence of the coding tag, or a complement thereof, and all or a portion of the sequence of the nucleic acid bound to the capture agent, or a complement thereof; and

(c) visualizing a spatial location of the nucleic acid in the tissue section corresponding to the determined sequence.

2. The method of claim 1 , wherein the capture agent is directly immobilized on the substrate.

3. The method of claim 1 , wherein the capture agent is indirectly immobilized on the substrate.

4. The method of claim 1 , wherein the capture agent is immobilized in a well on the substrate.

5. The method of claim 1 , wherein the substrate is incorporated into a flow cell.

6. The method of claim 1 , wherein the substrate is incorporated onto a slide.

7. The method of claim 1 , wherein the determining comprises sequencing.

8. The method of claim 1 , wherein the nucleic acid is DNA.

9. The method of claim 1 , wherein the nucleic acid is RNA.

10. The method of claim 1 , wherein the plurality of features comprises wells.

11. The method of claim 1 , wherein the tissue section is a fresh frozen tissue section.

12. The method of claim 1 , wherein the tissue section is a formalin fixed paraffin embedded tissue section.

13. The method of claim 1 , wherein the method further comprises staining the tissue section.

14. The method of claim 1 , wherein the method further comprises imaging the tissue section.

15. The method of claim 1 , wherein the substrate comprises greater than 10,000 features.

16. The method of claim 1 , wherein the capture agent further comprises a primer binding site.

17. The method of claim 1 , wherein multiple tissue sections are contacted with the substrate.

18. The method of claim 1 , wherein the method further comprises determining the abundance of the nucleic acid in the tissue section.

19. The method of claim 1 , wherein the tissue section is permeabilized prior to step (b).

20. A method for spatial analysis comprising:

(a) contacting a tissue section with a substrate of a flow cell, wherein the substrate comprises a plurality of wells, wherein a well of the plurality of wells comprises a capture agent immobilized therein, and wherein the capture agent comprises:

(i) a first sequence that binds a nucleic acid of the tissue section, and

(ii) a second sequence comprising a coding tag, wherein a sequence of the coding tag corresponds to a location of the capture agent on the substrate;

wherein nucleic acids of the tissue section are bound to capture agents at locations of the substrate that correspond to locations of the nucleic acids in the tissue section;

(b) determining the sequence of the coding tag, or a complement thereof, and all or a portion of the sequence of the nucleic acid bound to the capture agent, or a complement thereof; and

(c) visualizing a spatial location of the nucleic acids in the tissue section corresponding to the determined sequences.

21. The method of claim 20 , wherein the capture agent is directly immobilized in the well of the substrate.

22. The method of claim 20 , wherein the capture agent is indirectly immobilized in the well of the substrate.

23. The method of claim 20 , wherein the determining comprises sequencing.

24. The method of claim 20 , wherein the nucleic acid is RNA.

25. The method of claim 20 , wherein the tissue section is a fresh frozen tissue section.

26. The method of claim 20 , wherein the method further comprises staining the tissue section.

27. The method of claim 20 , wherein the method further comprises imaging the tissue section.

28. The method of claim 20 , wherein the capture agent further comprises a primer binding site.

29. The method of claim 20 , wherein multiple tissue sections are contacted with the substrate.

30. The method of claim 20 , wherein the method further comprises determining the abundance of the nucleic acids in the tissue section.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 27, 2024
From: CHEE, MARK S.
To: PROGNOSYS BIOSCIENCES, INC.
Reel/Frame 069689/0683 →
Continuity (13)
Continuation 18100127 · Jan 23, 2023
Continuation 17878519 · Aug 1, 2022
Continuation 17556588 · Dec 20, 2021
Continuation 17223669 · Apr 6, 2021
Continuation 17030230 · Sep 23, 2020
Continuation 16988284 · Aug 7, 2020
Continuation 16414213 · May 16, 2019
Continuation 16402098 · May 2, 2019
Continuation 16276235 · Feb 14, 2019
Continuation 15187661 · Jun 20, 2016
Continuation 13080616 · Apr 5, 2011
Provisional Application 61321124 · Apr 5, 2010
Related Publication 20250101502A1 · Mar 27, 2025
References Cited (400)
US 4514388A · Psaledakis · 1985 [cited by applicant]
US 4557903A · McCormick · 1985 [cited by applicant]
US 4574729A · Wells · 1986 [cited by applicant]
US 4683195A · Mullis · 1987 [cited by applicant]
US 4683202A · Mullis · 1987 [cited by applicant]
US 4731335A · Brigati · 1988 [cited by applicant]
US 4800159A · Mullis · 1989 [cited by applicant]
US 4883867A · Lee · 1989 [cited by applicant]
US 4965188A · Mullis · 1990 [cited by applicant]
US 4968601A · Jacobson et al. · 1990 [cited by applicant]
US 4988617A · Landegren et al. · 1991 [cited by applicant]
US 5002882A · Lunnen · 1991 [cited by applicant]
US 5061049A · Hornbeck · 1991 [cited by applicant]
US 5130238A · Malek · 1992 [cited by applicant]
US 5183053A · Yeh et al. · 1993 [cited by applicant]
US 5308751A · Ohkawa · 1994 [cited by applicant]
US 5321130A · Yue · 1994 [cited by applicant]
US 5410030A · Yue · 1995 [cited by applicant]
US 5436129A · Stapleton · 1995 [cited by applicant]
US 5436134A · Haugland · 1995 [cited by applicant]
US 5455166A · Walker · 1995 [cited by applicant]
US 5472881A · Beebe et al. · 1995 [cited by applicant]
US 5487972A · Gelfand et al. · 1996 [cited by applicant]
US 5494810A · Barany et al. · 1996 [cited by applicant]
US 5496518A · Arai et al. · 1996 [cited by applicant]
US 5503980A · Cantor · 1996 [cited by applicant]
US 5512439A · Hornes · 1996 [cited by applicant]
US 5512462A · Cheng · 1996 [cited by applicant]
US 5512478A · Orser et al. · 1996 [cited by applicant]
US 5559032A · Porneroy · 1996 [cited by applicant]
US 5582977A · Yue · 1996 [cited by applicant]
US 5589173A · O'Brien · 1996 [cited by applicant]
US 5599675A · Brenner · 1997 [cited by applicant]
US 5610287A · Nikiforov et al. · 1997 [cited by applicant]
US 5641658A · Adams · 1997 [cited by applicant]
US 5648245A · Fire et al. · 1997 [cited by applicant]
US 5658751A · Yue · 1997 [cited by applicant]
US 5667976A · Van Ness et al. · 1997 [cited by applicant]
US 5672344A · Kelley et al. · 1997 [cited by applicant]
US 5695940A · Drmanac et al. · 1997 [cited by applicant]
US 5716825A · Hancock et al. · 1998 [cited by applicant]
US 5750341A · Macevicz · 1998 [cited by applicant]
US 5752982A · Lang et al. · 1998 [cited by applicant]
US 5763175A · Brenner · 1998 [cited by applicant]
US 5807522A · Brown et al. · 1998 [cited by applicant]
US 5817783A · Calabretta et al. · 1998 [cited by applicant]
US 5830711A · Barany et al. · 1998 [cited by applicant]
US 5837832A · Chee et al. · 1998 [cited by applicant]
US 5837860A · Anderson et al. · 1998 [cited by applicant]
US 5854033A · Lizardi · 1998 [cited by applicant]
US 5863753A · Haugland · 1999 [cited by applicant]
US 5866377A · Kim et al. · 1999 [cited by applicant]
US 5871921A · Landegren et al. · 1999 [cited by applicant]
US 5875258A · Ortyn et al. · 1999 [cited by applicant]
US 5912148A · Eggerding · 1999 [cited by applicant]
US 5919626A · Shi et al. · 1999 [cited by applicant]
US 5925545A · Reznikoff et al. · 1999 [cited by applicant]
US 5928906A · Koester et al. · 1999 [cited by applicant]
US 5958775A · Wickstrom et al. · 1999 [cited by applicant]
US 5962271A · Chenchik et al. · 1999 [cited by applicant]
US 5962272A · Chenchik et al. · 1999 [cited by applicant]
US 5965443A · Reznikoff et al. · 1999 [cited by applicant]
US 5994136A · Naldini et al. · 1999 [cited by applicant]
US 6013440A · Lipshutz · 2000 [cited by applicant]
US 6013516A · Verma et al. · 2000 [cited by applicant]
US 6027889A · Barany et al. · 2000 [cited by applicant]
US 6054274A · Sampson et al. · 2000 [cited by applicant]
US 6060240A · Kamb et al. · 2000 [cited by applicant]
US 6083761A · Kedar et al. · 2000 [cited by applicant]
US 6130073A · Eggerding · 2000 [cited by applicant]
US 6133436A · Koester et al. · 2000 [cited by applicant]
US 6136592A · Leighton · 2000 [cited by applicant]
US 6143496A · Brown · 2000 [cited by applicant]
US 6153389A · Haarer · 2000 [cited by applicant]
US 6153408A · Abastado et al. · 2000 [cited by applicant]
US 6157432A · Helbing · 2000 [cited by applicant]
US 6159736A · Reznikoff et al. · 2000 [cited by applicant]
US 6165714A · Lane et al. · 2000 [cited by applicant]
US 6172218B1 · Brenner · 2001 [cited by applicant]
US 6210891B1 · Nyren · 2001 [cited by applicant]
US 6210894B1 · Brennan · 2001 [cited by applicant]
US 6214587B1 · Dattagupta · 2001 [cited by applicant]
US 6221591B1 · Aerts · 2001 [cited by applicant]
US 6221654B1 · Quake · 2001 [cited by applicant]
US 6251639B1 · Kurn · 2001 [cited by applicant]
US 6258558B1 · Szostak · 2001 [cited by applicant]
US 6258568B1 · Nyren · 2001 [cited by applicant]
US 6261804B1 · Szostak · 2001 [cited by applicant]
US 6265552B1 · Schatz · 2001 [cited by applicant]
US 6266459B1 · Walt · 2001 [cited by applicant]
US 6268148B1 · Barany et al. · 2001 [cited by applicant]
US 6274320B1 · Rothberg · 2001 [cited by applicant]
US 6281804B1 · Haller · 2001 [cited by applicant]
US 6291180B1 · Chu · 2001 [cited by applicant]
US 6291187B1 · Kingsmore et al. · 2001 [cited by applicant]
US 6300063B1 · Lipshutz et al. · 2001 [cited by applicant]
US 6300093B1 · Kindsvogel et al. · 2001 [cited by applicant]
US 6306597B1 · Macevicz · 2001 [cited by applicant]
US 6309824B1 · Drmanac · 2001 [cited by applicant]
US 6323009B1 · Lasken et al. · 2001 [cited by applicant]
US 6337472B1 · Garner et al. · 2002 [cited by applicant]
US 6344316B1 · Lockhart · 2002 [cited by applicant]
US 6344329B1 · Lizardi et al. · 2002 [cited by applicant]
US 6348990B1 · Igasaki et al. · 2002 [cited by applicant]
US 6355431B1 · Chee · 2002 [cited by applicant]
US 6368801B1 · Faruqi · 2002 [cited by applicant]
US 6391937B1 · Beuhler et al. · 2002 [cited by applicant]
US 6401267B1 · Drmanac · 2002 [cited by applicant]
US 6404907B1 · Gilchrist · 2002 [cited by applicant]
US 6416950B1 · Lohse · 2002 [cited by applicant]
US 6426215B1 · Sandell · 2002 [cited by applicant]
US 6432360B1 · Church et al. · 2002 [cited by applicant]
US 6444661B1 · Barton et al. · 2002 [cited by applicant]
US 6485982B1 · Charlton · 2002 [cited by applicant]
US 6503713B1 · Rana · 2003 [cited by applicant]
US 6506561B1 · Cheval et al. · 2003 [cited by applicant]
US 6518018B1 · Szostak · 2003 [cited by applicant]
US 6534266B1 · Singer · 2003 [cited by applicant]
US 6544732B1 · Chee · 2003 [cited by applicant]
US 6544790B1 · Sabatini · 2003 [cited by applicant]
US 6565727B1 · Shenderov · 2003 [cited by applicant]
US 6573043B1 · Cohen et al. · 2003 [cited by applicant]
US 6579695B1 · Lambalot · 2003 [cited by applicant]
US 6620584B1 · Chee · 2003 [cited by applicant]
US 6632641B1 · Brennan · 2003 [cited by applicant]
US 6673620B1 · Loeffler · 2004 [cited by applicant]
US 6677160B1 · Stockman et al. · 2004 [cited by applicant]
US 6699710B1 · Kononen · 2004 [cited by applicant]
US 6737236B1 · Pieken et al. · 2004 [cited by applicant]
US 6770441B2 · Dickinson · 2004 [cited by applicant]
US 6773566B2 · Shenderov · 2004 [cited by applicant]
US 6773886B2 · Kaufman · 2004 [cited by applicant]
US 6787308B2 · Balasubramanian · 2004 [cited by applicant]
US 6797470B2 · Barany et al. · 2004 [cited by applicant]
US 6800453B2 · Labaer · 2004 [cited by applicant]
US 6812005B2 · Fan et al. · 2004 [cited by applicant]
US 6828100B1 · Ronaghi · 2004 [cited by applicant]
US 6833246B2 · Balasubramanian · 2004 [cited by applicant]
US 6852487B1 · Barany et al. · 2005 [cited by applicant]
US 6859570B2 · Walt · 2005 [cited by applicant]
US 6864052B1 · Drmanac · 2005 [cited by applicant]
US 6867028B2 · Janulaitis · 2005 [cited by applicant]
US 6872816B1 · Hall et al. · 2005 [cited by applicant]
US 6875572B2 · Prudent et al. · 2005 [cited by applicant]
US 6878515B1 · Landegren · 2005 [cited by applicant]
US 6890741B2 · Fan et al. · 2005 [cited by applicant]
US 6897023B2 · Fu · 2005 [cited by applicant]
US 6911132B2 · Pamula · 2005 [cited by applicant]
US 6911345B2 · Quake · 2005 [cited by applicant]
US 6913881B1 · Aizenstein et al. · 2005 [cited by applicant]
US 6913921B2 · Fischer · 2005 [cited by applicant]
US 6942968B1 · Dickinson et al. · 2005 [cited by applicant]
US 6969488B2 · Bridgham · 2005 [cited by applicant]
US 6969589B2 · Patil · 2005 [cited by applicant]
US 6977033B2 · Becker · 2005 [cited by applicant]
US 7001792B2 · Sauer et al. · 2006 [cited by applicant]
US 7011944B2 · Prudent et al. · 2006 [cited by applicant]
US 7052244B2 · Fouillet · 2006 [cited by applicant]
US 7057026B2 · Barnes · 2006 [cited by applicant]
US 7060431B2 · Chee et al. · 2006 [cited by applicant]
US 7083980B2 · Reznikoff et al. · 2006 [cited by applicant]
US 7098041B2 · Kaylor et al. · 2006 [cited by applicant]
US 7115400B1 · Adessi · 2006 [cited by applicant]
US 7118883B2 · Inoue · 2006 [cited by applicant]
US 7128893B2 · Leamon et al. · 2006 [cited by applicant]
US 7163612B2 · Sterling · 2007 [cited by applicant]
US 7166431B2 · Chee et al. · 2007 [cited by applicant]
US 7192735B2 · Lambalot · 2007 [cited by applicant]
US 7211414B2 · Hardin · 2007 [cited by applicant]
US 7223371B2 · Hayenga et al. · 2007 [cited by applicant]
US 7229769B2 · Kozlov · 2007 [cited by applicant]
US 7244559B2 · Rothberg · 2007 [cited by applicant]
US 7255994B2 · Lao · 2007 [cited by applicant]
US 7258976B2 · Mitsuhashi · 2007 [cited by applicant]
US 7259258B2 · Kozlov et al. · 2007 [cited by applicant]
US 7264929B2 · Rothberg · 2007 [cited by applicant]
US 7270950B2 · Szostak · 2007 [cited by applicant]
US 7282328B2 · Kong et al. · 2007 [cited by applicant]
US 7297518B2 · Quake · 2007 [cited by applicant]
US 7315019B2 · Turner · 2008 [cited by applicant]
US 7328979B2 · Decre · 2008 [cited by applicant]
US 7329492B2 · Hardin · 2008 [cited by applicant]
US 7358047B2 · Hafner et al. · 2008 [cited by applicant]
US 7361488B2 · Fan et al. · 2008 [cited by applicant]
US 7375234B2 · Sharpless et al. · 2008 [cited by applicant]
US 7378242B2 · Hurt · 2008 [cited by applicant]
US 7393665B2 · Brenner · 2008 [cited by applicant]
US 7405281B2 · Xu · 2008 [cited by applicant]
US 7407757B2 · Brenner · 2008 [cited by applicant]
US 7427678B2 · Pieken et al. · 2008 [cited by applicant]
US 7456012B2 · Ryttsen et al. · 2008 [cited by applicant]
US 7462449B2 · Quake · 2008 [cited by applicant]
US 7473767B2 · Dimitrov · 2009 [cited by applicant]
US 7499806B2 · Kermani et al. · 2009 [cited by applicant]
US 7500637B2 · Marimon et al. · 2009 [cited by applicant]
US 7501245B2 · Quake · 2009 [cited by applicant]
US 7534991B2 · Miller et al. · 2009 [cited by applicant]
US 7537897B2 · Brenner · 2009 [cited by applicant]
US 7544473B2 · Brennan · 2009 [cited by applicant]
US 7547380B2 · Velev · 2009 [cited by applicant]
US 7555155B2 · Levenson et al. · 2009 [cited by applicant]
US 7561336B2 · Osaka et al. · 2009 [cited by applicant]
US 7563576B2 · Chee · 2009 [cited by applicant]
US 7579153B2 · Brenner · 2009 [cited by applicant]
US 7582420B2 · Oliphant et al. · 2009 [cited by applicant]
US 7595883B1 · El Gamal · 2009 [cited by applicant]
US 7601492B2 · Fu et al. · 2009 [cited by applicant]
US 7601498B2 · Mao · 2009 [cited by applicant]
US 7608434B2 · Reznikoff et al. · 2009 [cited by applicant]
US 7611869B2 · Fan · 2009 [cited by applicant]
US 7635566B2 · Brenner · 2009 [cited by applicant]
US 7641779B2 · Becker · 2010 [cited by applicant]
US 7655898B2 · Miller · 2010 [cited by applicant]
US 7666612B2 · Johnsson · 2010 [cited by applicant]
US 7674589B2 · Cohen et al. · 2010 [cited by applicant]
US 7674752B2 · He · 2010 [cited by applicant]
US 7700286B2 · Stroun et al. · 2010 [cited by applicant]
US 7709198B2 · Luo et al. · 2010 [cited by applicant]
US 7741106B2 · Moyle et al. · 2010 [cited by applicant]
US 7754429B2 · Rigatti · 2010 [cited by applicant]
US 7776547B2 · Roth · 2010 [cited by applicant]
US 7776567B2 · Mao · 2010 [cited by applicant]
US 7785869B2 · Belgrader et al. · 2010 [cited by applicant]
US 7803943B2 · Mao · 2010 [cited by applicant]
US 7844940B2 · Shin et al. · 2010 [cited by applicant]
US 7848553B2 · Hertel et al. · 2010 [cited by applicant]
US 7858321B2 · Glezer · 2010 [cited by applicant]
US 7888009B2 · Barany et al. · 2011 [cited by applicant]
US 7892747B2 · Barany et al. · 2011 [cited by applicant]
US 7910304B2 · Drmanac · 2011 [cited by applicant]
US 7914981B2 · Barany et al. · 2011 [cited by applicant]
US 7941279B2 · Hwang et al. · 2011 [cited by applicant]
US 7955794B2 · Shen et al. · 2011 [cited by applicant]
US 7960119B2 · Chee · 2011 [cited by applicant]
US 7960120B2 · Rigatti · 2011 [cited by applicant]
US 7985565B2 · Mayer et al. · 2011 [cited by applicant]
US 8003354B2 · Shen et al. · 2011 [cited by applicant]
US 8030477B2 · Cerrina et al. · 2011 [cited by applicant]
US 8046043B2 · Asano et al. · 2011 [cited by applicant]
US 8076063B2 · Fan · 2011 [cited by applicant]
US 8092784B2 · Mao · 2012 [cited by applicant]
US 8124751B2 · Pierce et al. · 2012 [cited by applicant]
US 8131476B2 · Cline et al. · 2012 [cited by applicant]
US 8148068B2 · Brenner · 2012 [cited by applicant]
US 8148518B2 · Buchanan · 2012 [cited by applicant]
US 8198028B2 · Rigatti et al. · 2012 [cited by applicant]
US 8199999B2 · Hoyt et al. · 2012 [cited by applicant]
US 8206917B2 · Chee · 2012 [cited by applicant]
US 8207093B2 · Szostak · 2012 [cited by applicant]
US 8268554B2 · Schallmeiner · 2012 [cited by applicant]
US 8278034B2 · Muraca · 2012 [cited by applicant]
US 8288103B2 · Oliphant · 2012 [cited by applicant]
US 8288122B2 · O'Leary et al. · 2012 [cited by applicant]
US 8330087B2 · Domenicali · 2012 [cited by applicant]
US 8337851B2 · Aukerman · 2012 [cited by applicant]
US 8343500B2 · Wraith · 2013 [cited by applicant]
US 8383338B2 · Kitzman · 2013 [cited by applicant]
US 8415102B2 · Geiss et al. · 2013 [cited by applicant]
US 8431691B2 · McKernan et al. · 2013 [cited by applicant]
US 8460865B2 · Chee · 2013 [cited by applicant]
US 8462981B2 · Determan et al. · 2013 [cited by applicant]
US 8481257B2 · Van Eijk · 2013 [cited by applicant]
US 8481258B2 · Church et al. · 2013 [cited by applicant]
US 8481292B2 · Casbon · 2013 [cited by applicant]
US 8481698B2 · Lieberman et al. · 2013 [cited by applicant]
US 8486625B2 · Gunderson · 2013 [cited by applicant]
US 8507204B2 · Pierce et al. · 2013 [cited by applicant]
US 8519115B2 · Webster et al. · 2013 [cited by applicant]
US 8551710B2 · Bernitz et al. · 2013 [cited by applicant]
US 8568979B2 · Stuelpnagel et al. · 2013 [cited by applicant]
US RE44596E · Stroun et al. · 2013 [cited by applicant]
US 8586310B2 · Mitra · 2013 [cited by applicant]
US 8597891B2 · Barany et al. · 2013 [cited by applicant]
US 8603743B2 · Liu et al. · 2013 [cited by applicant]
US 8604182B2 · Luo et al. · 2013 [cited by applicant]
US 8614073B2 · Van Eijk · 2013 [cited by applicant]
US 8624016B2 · Barany et al. · 2014 [cited by applicant]
US 8637242B2 · Shen · 2014 [cited by applicant]
US 8658361B2 · Wu et al. · 2014 [cited by applicant]
US 8685889B2 · Van Eijk · 2014 [cited by applicant]
US 8741564B2 · Seligmann · 2014 [cited by applicant]
US 8741606B2 · Casbon · 2014 [cited by applicant]
US 8748103B2 · Faham et al. · 2014 [cited by applicant]
US 8771950B2 · Church et al. · 2014 [cited by applicant]
US 8778849B2 · Bowen · 2014 [cited by applicant]
US 8785353B2 · Van Eijk · 2014 [cited by applicant]
US 8790873B2 · Namsaraev et al. · 2014 [cited by applicant]
US 8809238B2 · Livak et al. · 2014 [cited by applicant]
US 8815512B2 · Van Eijk · 2014 [cited by applicant]
US 8835358B2 · Fodor · 2014 [cited by applicant]
US 8865410B2 · Shendure · 2014 [cited by applicant]
US 8906626B2 · Oliphant et al. · 2014 [cited by applicant]
US 8911945B2 · Van Eijk · 2014 [cited by applicant]
US 8936912B2 · Mitra · 2015 [cited by applicant]
US 8951726B2 · Luo et al. · 2015 [cited by applicant]
US 8951728B2 · Rasmussen · 2015 [cited by applicant]
US 8951781B2 · Reed · 2015 [cited by applicant]
US 8986926B2 · Ferree et al. · 2015 [cited by applicant]
US 9005891B2 · Sinicropi et al. · 2015 [cited by applicant]
US 9005935B2 · Belyaev · 2015 [cited by applicant]
US 9023768B2 · Van Eijk · 2015 [cited by applicant]
US 9062348B1 · Van Eijk · 2015 [cited by applicant]
US 9080210B2 · Van Eijk · 2015 [cited by applicant]
US 9085798B2 · Chee · 2015 [cited by applicant]
US 9121069B2 · Lo · 2015 [cited by applicant]
US 9163283B2 · Chee et al. · 2015 [cited by applicant]
US 9194001B2 · Brenner · 2015 [cited by applicant]
US 9201063B2 · Sood et al. · 2015 [cited by applicant]
US 9217176B2 · Faham et al. · 2015 [cited by applicant]
US 9290808B2 · Fodor · 2016 [cited by applicant]
US 9290809B2 · Fodor · 2016 [cited by applicant]
US 9309556B2 · Myllykangas et al. · 2016 [cited by applicant]
US 9328383B2 · Van Eijk · 2016 [cited by applicant]
US 9334536B2 · Van Eijk · 2016 [cited by applicant]
US 9340830B2 · Lipson · 2016 [cited by applicant]
US 9371563B2 · Geiss et al. · 2016 [cited by applicant]
US 9371598B2 · Chee · 2016 [cited by applicant]
US 9376716B2 · Van Eijk · 2016 [cited by applicant]
US 9376717B2 · Gao et al. · 2016 [cited by applicant]
US 9376719B2 · Van Eijk · 2016 [cited by applicant]
US 9404156B2 · Hicks · 2016 [cited by applicant]
US 9416409B2 · Hayden · 2016 [cited by applicant]
US 9447459B2 · Van Eijk · 2016 [cited by applicant]
US 9453256B2 · Van Eijk · 2016 [cited by applicant]
US 9493820B2 · Van Eijk · 2016 [cited by applicant]
US 9494588B2 · Springer · 2016 [cited by applicant]
US 9506061B2 · Brown · 2016 [cited by applicant]
US 9512487B2 · Faham et al. · 2016 [cited by applicant]
US 9541504B2 · Hoyt · 2017 [cited by applicant]
US 9557330B2 · Siciliano et al. · 2017 [cited by applicant]
US 9574230B2 · Van Eijk · 2017 [cited by applicant]
US 9593365B2 · Frisen et al. · 2017 [cited by applicant]
US 9598728B2 · Barany et al. · 2017 [cited by applicant]
US 9624538B2 · Church et al. · 2017 [cited by applicant]
US 9657335B2 · Van Eijk · 2017 [cited by applicant]
US 9670542B2 · Van Eijk · 2017 [cited by applicant]
US 9671344B2 · Staker · 2017 [cited by applicant]
US 9694361B2 · Bharadwaj · 2017 [cited by applicant]
US 9702004B2 · Van Eijk · 2017 [cited by applicant]
US 9714446B2 · Webster et al. · 2017 [cited by applicant]
US 9714937B2 · Dunaway · 2017 [cited by applicant]
US 9745627B2 · Van Eijk · 2017 [cited by applicant]
US 9777324B2 · Van Eijk · 2017 [cited by applicant]
US 9816134B2 · Namsaraev · 2017 [cited by applicant]
US 9850536B2 · Oliphant et al. · 2017 [cited by applicant]
US 9868979B2 · Chee et al. · 2018 [cited by applicant]
US 9879313B2 · Chee et al. · 2018 [cited by applicant]
US 9889422B2 · Smith et al. · 2018 [cited by applicant]
US 9896721B2 · Van Eijk · 2018 [cited by applicant]
US 9898576B2 · Van Eijk · 2018 [cited by applicant]
US 9898577B2 · Van Eijk · 2018 [cited by applicant]
US 9902950B2 · Church et al. · 2018 [cited by applicant]
US 9902991B2 · Sinicropi et al. · 2018 [cited by applicant]
US 9958454B2 · Kozlov et al. · 2018 [cited by applicant]
US 10023907B2 · Van Eijk · 2018 [cited by applicant]
US 10030261B2 · Frisen et al. · 2018 [cited by applicant]
US 10072104B2 · Winnik et al. · 2018 [cited by applicant]
US 10077478B2 · Faham et al. · 2018 [cited by applicant]
US 10095832B2 · Van Eijk · 2018 [cited by applicant]
US 10144966B2 · Cantor · 2018 [cited by applicant]
US 10196691B2 · Harkin et al. · 2019 [cited by applicant]
US 10227639B2 · Levner et al. · 2019 [cited by applicant]
US 10246752B2 · Faham et al. · 2019 [cited by applicant]
US 10266876B2 · Cai et al. · 2019 [cited by applicant]
US 10267808B2 · Cai · 2019 [cited by applicant]
US 10357771B2 · Bharadwaj · 2019 [cited by applicant]
US 10391467B2 · Zhou et al. · 2019 [cited by applicant]
US 10428326B2 · Belhocine et al. · 2019 [cited by applicant]
US 10465235B2 · Gullberg et al. · 2019 [cited by applicant]
US 10501791B2 · Church et al. · 2019 [cited by applicant]
US 10633648B2 · Seelig et al. · 2020 [cited by applicant]
US 10669569B2 · Gullberg et al. · 2020 [cited by applicant]
US 10685210B2 · Wimberger-Friedl et al. · 2020 [cited by applicant]
US 10697013B1 · Brenner et al. · 2020 [cited by applicant]
US 10725027B2 · Bell · 2020 [cited by applicant]
US 10767223B1 · Brenner et al. · 2020 [cited by applicant]
US 10774372B2 · Chee et al. · 2020 [cited by applicant]
US 10774374B2 · Frisen et al. · 2020 [cited by applicant]
US 10829803B2 · Terbrueggen et al. · 2020 [cited by applicant]
US 10858702B2 · Lucero et al. · 2020 [cited by applicant]
US 10927403B2 · Chee et al. · 2021 [cited by applicant]
US 10995362B2 · Dallett et al. · 2021 [cited by applicant]
US 11046996B1 · Chee et al. · 2021 [cited by applicant]
US 11162132B2 · Frisen et al. · 2021 [cited by applicant]
US 11214796B2 · Shirai et al. · 2022 [cited by applicant]
US 11286515B2 · Chee et al. · 2022 [cited by applicant]
US 11299774B2 · Frisen et al. · 2022 [cited by applicant]
US 11332790B2 · Chell et al. · 2022 [cited by applicant]
US 11352659B2 · Frisen et al. · 2022 [cited by applicant]
US 11359228B2 · Chee et al. · 2022 [cited by applicant]
US 11390912B2 · Frisen et al. · 2022 [cited by applicant]
US 11407992B2 · Dadhwal · 2022 [cited by applicant]
US 11408029B2 · Katiraee et al. · 2022 [cited by applicant]
US 11434524B2 · Ramachandran Iyer et al. · 2022 [cited by applicant]
US 11459607B1 · Terry et al. · 2022 [cited by applicant]
US 11479809B2 · Frisen et al. · 2022 [cited by applicant]
US 11492612B1 · Dadhwal · 2022 [cited by applicant]
US 11501440B2 · Weisenfeld et al. · 2022 [cited by applicant]
US 11505828B2 · Chell et al. · 2022 [cited by applicant]
US 11512308B2 · Gallant et al. · 2022 [cited by applicant]
Cited By (18)
US 12,371,688 US 12,405,264 US 12,416,603 US 12,442,045 US 12,497,654 US 12,508,590 US 12,545,949 US 12,553,805 US 12,553,898 US 12,566,113 US 12,566,114 US 12,571,029 US 12,624,475 US 12,637,706 US 12,692,492 US 12,735,743 US 12,742,199 US 12,747,475