Amplification and detection reagents for HIV-1
Oligonucleotide primer sets, probes, and combinations of the primer sets and probes are provided herein. These reagents are useful for amplifying and detecting HIV-1 target sequences in a test sample.
1. A probe selected from the group consisting of:
SEQ ID NO: 41; SEQ ID NO: 42; SEQ ID NO: 43; SEQ ID NO: 44; SEQ ID NO: 45; SEQ ID NO: 46; SEQ ID NO: 47; SEQ ID NO: 48; SEQ ID NO: 49; SEQ ID NO: 58; SEQ ID NO: 59; SEQ ID NO: 60; SEQ ID NO: 61; SEQ ID NO: 62; SEQ ID NO: 63; SEQ ID NO: 64; and SEQ ID NO: 65.
2. The probe of claim 1 , wherein the probe is selected from the group consisting of: SEQ ID NO: 41; SEQ ID NO: 42; SEQ ID NO: 45; SEQ ID NO: 48; and SEQ ID NO: 49.
3. A method for detecting the presence of HIV-1 in a test sample comprising:
contacting a test sample with amplification reagents;
placing the reaction mixture under amplification conditions to form an amplification product;
forming a hybrid between the amplification product and a probe selected from the group consisting of SEQ ID NO: 41; SEQ ID NO: 42; SEQ ID NO: 43; SEQ ID NO: 44; SEQ ID NO: 45; SEQ ID NO: 46; SEQ ID NO: 47; SEQ ID NO: 48; SEQ ID NO: 49; SEQ ID NO: 58; SEQ ID NO: 59; SEQ ID NO: 60; SEQ ID NO: 61; SEQ ID NO: 62; SEQ ID NO: 63; SEQ ID NO: 64; and SEQ ID NO: 65; and
detecting the hybrid as an indication of the presence of HIV-1 in the test sample.
4. The method of claim 3 wherein the hybrid is formed while the reaction mixture is under amplification conditions.
5. The method of claim 3 ,
wherein the probe is selected from the group consisting of SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 46, SEQ ID NO: 47 and SEQ ID NO: 48, and
wherein the amplification reagents comprise SEQ ID NO: 28.
6. The method of claim 5 , wherein the amplification reagents include a primers selected from the group consisting of:
SEQ ID NO: 28 and SEQ ID NO: 29, SEQ ID NO: 28 and SEQ ID NO: 30, SEQ ID NO: 28 and SEQ ID NO: 31, SEQ ID NO: 28 and SEQ ID NO: 32, and SEQ ID NO: 28 and SEQ ID NO: 33.
7. The method of claim 3 , wherein the probe comprises SEQ ID NO: 49 and wherein the amplification reagents comprise SEQ ID NO: 28.
8. The method of claim 7 , wherein the amplification reagents include a primers selected from the group consisting of:
SEQ ID NO: 28 and SEQ ID NO: 29, SEQ ID NO: 28 and SEQ ID NO: 30, SEQ ID NO: 28 and SEQ ID NO: 32, and SEQ ID NO: 28 and SEQ ID NO: 33.