Phosphodiesterase 10
The present invention provides novel human PDE10 polypeptides, polynucleotides encoding the polypeptides, expression constructs comprising the polynucleotides, host cells transformed with the expression constructs; methods for producing PDE10 polypeptides; antisense polynucleotides; and antibodies specifically immunoreactive with the PDE10 polypeptides. The invention further provides methods to identify binding partners of PDE 10, and more particularly, binding partners that modulate PDE10 enzyme activity.
1. A method to identify a specific binding partner compound of a phosphodiesterase polypeptide encoded by a polynucleotide encoding the polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 2;
said method comprising the steps of:
a) contacting the phosphodiesterase polypeptide with a compound under conditions which permit binding between the compound and the phosphodiesterase polypeptide;
b) detecting binding of the compound to the phosphodiesterase polypeptide; and
c) identifying the compound as a specific binding partner of the phosphodiesterase polypeptide.
2. The method according to claim 1 wherein the specific binding partner modulates activity of the phosphodiesterase polypeptide.
3. The method according to claim 2 wherein the compound inhibits activity of the phosphodiesterase polypeptide.
4. The method according to claim 2 wherein the compound enhances activity of the phosphodiesterase polypeptide.