Pesticidal toxins
The subject invention concerns new classes of insecticidal proteins obtainable from Bacillus thuringiensis , and polynucleotides that encode these proteins. The subject invention also includes transgenic cells and plants that produce these proteins. The proteins are preferably in the 10-15 kDa and 40-50 kDa size range.
1. An isolated polynucleotide that encodes a protein that has toxin activity against a corn rootworm pest, wherein the full complement of the nucleotide sequence represented by SEQ ID NO:31 hybridizes under stringent conditions with a nucleic acid sequence that codes for said protein.
2. The polynucleotide of claim 1 wherein said protein has a molecular weight of between about 10 kDa and about 15 kDa.
3 .The polynucleotide of claim 1 wherein said stringent conditions comprise a wash in 1×SSPE and 0.1% SDS at room temperature.
4. The polynucleotide of claim 1 wherein said stringent conditions comprise a wash in 0.2×SSPE and 0.1% SDS at room temperature.
5. An isolated polynucleotide that encodes a protein that has toxin activity against a corn rootworm pest, wherein the frill complement of a nucleotide sequence selected from the group consisting of SEQ ID NO:35, SEQ ID NO:40, and SEQ ID NO:44 hybridizes under stringent conditions with a nucleic acid sequence that codes for said protein.
6. The polynucleotide of claim 5 wherein said nucleotide sequence is SEQ ID NO:35.
7. The polynucleotide of claim 5 wherein said nucleotide sequence is SEQ ID NO:40.
8. The polynucleotide of claim 5 wherein said nucleotide sequence is SEQ ID NO:44.
9. An isolated polynucleotide that encodes a protein that has toxin activity against a corn rootworm pest, wherein the full complement of a nucleotide sequence that codes for the amino acid sequence represented by SEQ ID NO:32 hybridizes under stringent conditions with a nucleic acid sequence that codes for said protein.
10. The polynucleotide of claim 9 wherein said protein has a molecular weight of between about 10 kDa and about 15 kDa.
11. The polynucleotide of claim 9 wherein said stringent conditions comprise a wash in 1×SSPE and 0.1% SDS at room temperature.
12. The polynucleotide of claim 9 wherein said stringent conditions comprise a wash in 0.2×SSPE and 0.1% SDS at room temperature.
13. An isolated polynucleotide that encodes a protein that has toxin activity against a corn rootworm pest, wherein the full complement of a nucleotide sequence that codes for the amino acid sequence selected from the group consisting of SEQ ID NO:36 and SEQ ID NQ:41 hybridizes under stringent conditions with a nucleic acid sequence that codes for said protein.
14. The polynucleotide of claim 13 wherein said amino acid sequence is SEQ ID NO:36.
15. The polynucleotide of claim 13 wherein said amino acid sequence is SEQ ID NO:41.
16. A transgenic cell comprising an isolated polynucleotide of claim 1 .
17. A transgenic cell comprising an isolated polynucleotide of claim 5 .
18. A transgenic cell comprising an isolated polynucleotide of claim 9 .
19. A transgenic cell comprising an isolated polynucleotide of claim 13 .
20. The transgenic cell of claim 16 wherein said cell is a microbial cell.
21. The transgenic cell of claim 16 wherein said cell is a bacterial cell.
22. The transgenic cell of claim 16 wherein said cell is a plant cell.
23. The transgenic cell of claim 16 wherein said cell is a cell of a corn plant.
24. The transgenic cell of claim 16 wherein said cell is a corn root cell.