Complex combinatorial chemical libraries encoded with tags
Encoded combinatorial chemistry is provided, where sequential synthetic schemes are recorded using organic molecules, which define choice of reactant, and stage, as the same or different bit of information. Various products can be produced in the multi-stage synthesis, such as oligomers and synthetic non-repetitive organic molecules. Conveniently, nested families of compounds can be employed as identifiers, where number and/or position of a substituent define the choice. Alternatively, detectable functionalities may be employed, such as radioisotopes, fluorescers, halogens, and the like, where presence and ratios of two different groups can be used to define stage or choice. Particularly, pluralities of identifiers may be used to provide a binary or higher code, so as to define a plurality of choices with only a few detachable tags. The particles may be screened for a characteristic of interest, particularly binding affinity, where the products may be detachable from the particle or retained on the particle. The reaction history of the particles which are positive for the characteristic can be determined by the release of the tags and analysis to define the reaction history of the particle.
1. A method of determining the structure of a compound, which comprises:
a) providing a solid support on which a compound was synthesized by a reaction series comprising at least a first stage reagent and/or first stage reaction condition, and a second stage reagent and/or second stage reaction condition, wherein the first stage precedes the second stage in the reaction series, which solid support has attached thereto:
i. a first identifier having a first tag, which tag comprises a code that records the first stage reagent or the first stage reaction condition; and
ii. a second identifier having a second tag, which tag comprises a code that records the second stage reagent or the second stage reaction condition;
b) detaching the first tag and the second tag from the solid support such that a mixture of soluble tags is formed;
c) reacting the mixture of tags with a labeling reagent which introduces a detectable component to each tag;
d) detecting each tag via its detectable component;
e) determining the identity of each of the detected tags from step (d), whereby the identities of the reagents and/or reaction conditions of the first and second stages of the reaction series are determined: and
f) deriving the structure of a compound from the identities of the detected tags.
2. The method of claim 1 , wherein the labeling reagent is a halogenated aromatic, radioactive, a radioisotope or a chemiluminescer.
3. The method of claim 1 , wherein the labeling reagent is a fluorescer.
4. The method of claim 3 , wherein the labeling reagent is dansyl chloride.
5. The method of claim 1 , wherein said detecting is by fluorescence or electron capture.
6. The method of claim 1 , wherein the first tag and second tag are attached to the solid support through a cleavable linker.
7. The method of claim 1 , wherein the compound was synthesized as a member of a combinatorial library.
8. A method of determining the structure of a compound, which comprises:
a) providing a solid support on which a compound was synthesized by a reaction series comprising at least a first stage reagent and/or first stage reaction condition, and a second stage reagent and/or second stage reaction condition, wherein the first stage precedes the second stage in the reaction series, which solid support has attached thereto:
i. a first identifier having a first tag, which tag comprises a code that records the first stage reagent or the first stage reaction condition;
ii. and a second identifier having a second tag, which tag comprises a code that records the second stage reagent or the second stage reaction condition;
b) reacting the solid support with a labeling reagent which introduces a detectable component to each tag;
c) detaching the first tag and the second tag from the solid support such that a mixture of soluble tags is formed;
d) detecting each tag via its detectable component;
e) determining the identity of each of the detected tags from step (d), whereby the identities of the reagents and/or reaction conditions of the first and second stages of the reaction series are determined; and
f) deriving the structure of a compound from the identities of the detected tags.
9. The method of claim 8 , wherein the labeling reagent is a haloaromatic, a radioisotope or a chemiluminescer.
10. The method of claim 8 , wherein the labeling reagent is a fluorescer.
11. The method of claim 10 , wherein the labeling reagent is dansyl chloride.
12. The method of claim 8 , wherein said detecting is by fluorescence or electron capture.
13. The method of claim 8 , wherein the first tag and the second tag are attached to the solid support through a cleavable linker.
14. The method of claim 8 , wherein the compound was synthesized as a member of a combinatorial library.