Glycosyl sulfotransferase-3
View Patent ↗A novel human glycosylsulfotransferase expressed in high endothelial cells (GST-3) and polypeptides related thereto, as well as nucleic acid compositions encoding the same, are provided. The subject polypeptides and nucleic acid compositions find use in a variety of applications, including research, diagnostic, and therapeutic agent screening applications. Also provided are methods of inhibiting selectin mediated binding events and methods of treating disease conditions associated therewith.
1. An isolated nucleic acid molecule that hybridizes under stringent conditions to a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO:01, or its complementary sequence, wherein stringent hybridization conditions comprise hybridization at 50° C. or higher in a solution comprising 15 mM sodium chloride and 1.5 mM sodium citrate, wherein said nucleotide sequence encodes a polypeptide that catalyzes the transfer of a sulfate group from a sulfate donor to a sulfate acceptor.
2. An expression vector comprising the nucleic acid of claim 1 .
3. An isolated host cell comprising the expression vector of claim 2 .
4. The host cell of claim 3 , wherein the cell is prokaryotic.
5. The host cell of claim 3 , wherein the cell is eukaryotic.
6. A method of producing a glycosyl sulfotransferase-3 polypeptide, said method comprising:
growing a cell according to claim 3 , whereby said polypeptide is expressed; and
isolating said polypeptide.
7. The isolated nucleic acid of claim 1 , wherein said nucleic acid comprises the nucleotide sequence set forth in SEQ ID NO:1.
8. A composition comprising the nucleic acid of claim 1 .
9. The composition according to claim 8 , wherein said composition further comprises a test agent.
10. The composition according to claim 8 , wherein said composition further comprises a sulfate donor.
11. The composition according to claim 8 , wherein said composition further comprises a sulfate acceptor.
12. The composition according to claim 11 , wherein said sulfate acceptor is a selectin.
13. The expression vector of claim 2 , wherein said nucleic acid is operably linked to an exogenous control region.