OAT5 molecules and uses therefor
The invention provides isolated nucleic acids molecules, designated OAT5 nucleic acid molecules, which encode organic anion transporters. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing OAT5 nucleic acid molecules, host cells into which the expression vectors have been introduced, and non-human transgenic animals in which a OAT5 gene has been introduced or disrupted. The invention still further provides isolated OAT5 polypeptides, fusion polypeptides, antigenic peptides and anti-OAT5 antibodies. Diagnostic and therapeutic methods utilizing compositions of the invention are also provided.
1. An isolated nucleic acid molecule selected from the group consisting of:
a) a nucleic acid molecule comprising a nucleotide sequence which is at least 95% identical to the nucleotide sequence of SEQ ID NO:7 or 9, wherein the nucleic acid molecule encodes a polypeptide having organic anion transporter (OAT) activity;
b) a nucleic acid molecule comprising a fragment of at least 1200 contiguous nucleotides of SEQ ID NO:7 or 9, wherein the fragment encodes a polypeptide having OAT activity;
c) a nucleic acid molecule which encodes a polypeptide comprising an amino acid sequence which is at least 95% identical to the amino acid sequence of SEQ ID NO:8, wherein the polypeptide has OAT activity; and
d) a nucleic acid molecule which encodes a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:8, wherein the fragment comprises at least 400 contiguous amino acid residues of the amino acid sequence of SEQ ID NO:8 and has OAT activity.
2. An isolated nucleic acid molecule selected from the group consisting of:
a) a nucleic acid molecule comprising the nucleic acid sequence of SEQ ID NO:7 or SEQ ID NO:9;
b) a nucleic acid molecule consisting of the nucleic acid sequence of SEQ ID NO: 7 or SEQ ID NO:9;
c) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:8; and
d) a nucleic acid molecule which encodes a polypeptide consisting of the amino acid sequence of SEQ ID NO: 8.
3. The nucleic acid molecule of claim 1 , further comprising vector nucleic acid sequences.
4. The nucleic acid molecule of claim 2 , further comprising vector nucleic acid sequences.
5. The nucleic acid molecule of claim 1 , further comprising nucleic acid sequences encoding a heterologous polypeptide.
6. The nucleic acid molecule of claim 2 , further comprising nucleic acid sequences encoding a heterologous polypeptide.
7. An isolated host cell which contains the nucleic acid molecule of claim 3 .
8. The host cell of claim 7 which is a mammalian host cell.
9. An isolated host cell which contains the nucleic acid molecule of claim 4 .
10. The host cell of claim 9 which is a mammalian host cell.
11. A method for producing a polypeptide encoded by a nucleic acid molecule of claim 1 , comprising culturing a host cell containing the nucleic acid molecule of claim 1 under conditions in which the polypeptide is expressed, thereby producing the polypeptide.
12. A method for producing a polypeptide encoded by a nucleic acid molecule of claim 2 , comprising culturing a host cell containing the nucleic acid molecule of claim 2 under conditions in which the polypeptide is expressed, thereby producing the polypeptide.