Use of FXR ligands
The present invention includes nuclear receptor heterodimer and nuclear receptor-coactivator peptide assays for identifying ligands for nuclear receptors, utilizing scintillation proximity and fluorescence resonance energy transfer (FRET), and methods of using identified ligands.
1. A method of lowering serum triglycerides in a mammal comprising administering to a mammal in need thereof an effeetive serum triglyceride lowering amount of a nonsteroidal agonist for Farnesoid X Receptor of the following formula:
wherein X 1 is CH, N; X 2 is O or NH; R and R 1 are independently H, lower alkyl, halogen, or CF 3 ; R 2 is lower alkyl; R 3 and R 4 are independently H, lower alkyl, halogen, CF 3 , OH, O-alkyl, or O-polyhaloalkyl.
2. The method of claim 1 , wherein said FXR agonist has the following formula: