Encoded excitation source Raman spectroscopy methods and systems
Selected combinations from three or more light excitation wavelengths are serially impinged on a sample based on an encoding pattern. A plurality of spectra is detected from the sample responsive to respective ones of the selected combinations of light excitation wavelengths. A shifted Raman excitation spectral component and a non-shifted spectral component characteristic of the sample are identified based on the plurality of spectra.
1. A method for performing Raman spectroscopy comprising:
serially impinging selected combinations from three or more light excitation wavelengths on a sample based on an encoding pattern;
detecting a plurality of spectra from the sample responsive to the selected combinations of light excitation wavelengths; and
identifying a shifted Raman excitation spectral component and a non-shifted spectral component characteristic of the sample based on the plurality of spectra.
2. The method of claim 1 , wherein the selected combinations comprise multi-wavelength light excitation beams.
3. The method of claim 1 , wherein identifying a shifted Raman excitation spectral component and a non-shifted spectral component comprises comparing ones of the plurality of spectra and identifying components of the spectra that are shifted or non-shifted responsive to changes in wavelengths of the selected combinations.
4. The method of claim 1 , wherein the encoding pattern includes at least a portion of a Hadamard encoding pattern.
5. The method of claim 1 , wherein the encoding pattern includes at least a portion of a Walsh encoding pattern.
6. The method of claim 1 , wherein selectively impinging selected combinations from three or more light excitation wavelengths comprises selectively illuminating radiation source elements in an array of radiation source elements according to the encoding pattern.
7. The method of claim 6 , wherein selectively impinging selected combinations from three or more light excitation wavelengths comprises identifying a first subset and a second subset of non-overlapping radiation source elements from the array of radiation source elements and wherein the selected combinations comprise a first excitation beam including the first subset of radiation source elements and a second excitation beam including the second subset of radiation source elements.
8. The method of claim 7 , wherein identifying a shifted Raman excitation spectral component and a non-shifted spectral component comprises determining a difference between a spectrum responsive to the first excitation beam and a spectrum responsive to the second excitation beam.
9. The method of claim 7 , wherein the first subset and the second subset of radiation source elements include the same number of radiation source elements.
10. The method of claim 7 , further comprising selecting the first subset and the second subset of radiation source elements based on at least a portion of a Hadamard encoding pattern.
11. The method of claim 7 , wherein the first subset of radiation source elements is based on a row or column of a Hadamard matrix and the second subset of radiation source elements is based on an inverse of the row or column of the Hadamard matrix.
12. The method of claim 7 , wherein identifying a shifted Raman excitation spectral component and a non-shifted spectral component comprises analyzing the plurality of spectra using a Hadamard encoding pattern.
13. The method of claim 1 , wherein the non-shifted spectral component comprises fluorescence radiation.
14. The method of claim 6 , wherein the radiation source elements comprise laser emitting diodes or lasers.
15. The method of claim 2 , wherein the multi-wavelength light excitation beams include three or more wavelengths.
16. The method of claim 1 , wherein serially impinging selected combinations from three or more light excitation wavelengths comprises filtering a broadband excitation source to provide the selected wavelength combination.
17. A system for performing Raman spectroscopy comprising:
an electromagnetic radiation source configured to serially impinge selected combinations from three or more light excitation wavelengths on a sample based on an encoding pattern;
a detector configured to detect a plurality of spectra from the sample responsive to respective ones of the selected combinations; and
a signal analyzer in communication with the detector configured to receive the plurality of spectra and to identify a shifted Raman excitation spectral component and a non-shifted spectral component characteristic of the sample based on the plurality of spectra.
18. The system of claim 17 , wherein the selected combinations comprise multi-wavelength light excitation beams.
19. The system of claim 17 , wherein the signal analyzer is configured to identify a shifted Raman excitation spectral component and a non-shifted spectral component by comparing ones of the plurality of spectra and identifying components of the spectra that are shifted or non-shifted responsive to changes in wavelengths of the selected combinations.
20. The system of claim 17 , wherein the electromagnetic radiation source is configured to serially impinge the selected combinations of wavelengths based on an encoding pattern that includes at least a portion of a Hadamard encoding pattern.
21. The system of claim 17 , wherein the electromagnetic radiation source is configured to serially impinge the selected combinations of wavelengths based on an encoding pattern that includes at least a portion of a Walsh encoding pattern.
22. The system of claim 17 , wherein the electromagnetic radiation source comprises an array of radiation source elements and is configured to selectively impinge selected combinations from three or more light excitation wavelengths by selectively illuminating radiation source elements in the array of radiation source elements according to the encoding pattern.
23. The system of claim 22 , wherein the electromagnetic radiation source is configured to selectively impinge selected combinations from three or more light excitation wavelengths by identifying a first subset and a second subset of non-overlapping radiation source elements from the array of radiation source elements and wherein the selected combinations comprise a first excitation beam including the first subset of radiation source elements and a second excitation beam including the second subset of radiation source elements.
24. The system of claim 23 , wherein identifying a shifted Raman excitation spectral component and a non-shifted spectral component comprises determining a difference between a spectrum responsive to the first excitation beam and a spectrum responsive to the second excitation beam.
25. The system of claim 23 , wherein the first subset and the second subset of radiation source elements include the same number of radiation source elements.
26. The system of claim 23 , wherein the electromagnetic radiation source is further configured to select the first subset and the second subset of radiation source elements based on at least a portion of a Hadamard encoding pattern.
27. The system of claim 23 , the first subset of radiation source elements is based on a row or column of a Hadamard matrix and the second subset of radiation source elements is based on an inverse of the row or column of the Hadamard matrix.
28. The system of claim 23 , wherein the signal analyzer is configured to identify a shifted Raman excitation spectral component and a non-shifted spectral component by analyzing the plurality of spectra using a Hadamard encoding pattern.
29. The system of claim 17 , wherein the non-shifted spectral component comprises fluorescence radiation.
30. The system of claim 22 , wherein the radiation source elements comprise diodes or lasers.
31. The system of claim 18 , wherein the multi-wavelength light excitation beams include three or more wavelengths.