Concentration of protein and/or peptides samples
View Patent ↗The invention relates to a device for protein and/or peptide concentration, which device comprises electroconcentration means ( 23 ); at least two electrodes having a positive ( 7 ) and a negative charge ( 35 ), respectively; and protein and/or peptide capture means ( 17 ); wherein said electroconcentration means ( 23 ) comprises a funnel shaped cavity and at least one electrode is located on each side of the electroconcentration means. The invention also relates to a method for concentrating a protein and/or a peptide in a sample, which method can be performed in a device according to the invention.
1. A device for protein and/or peptide concentration in a sample, which device comprises
means for electroconcentrating ( 23 ) including a funnel shaped cavity ( 33 ) with a wide end and a narrow end;
at least two electrodes ( 7 , 35 ), one electrode ( 35 ) being positioned near to said wide end and one electrode ( 7 ) being positioned nearer to said narrow end;
one or more means for capturing proteins and/or peptides ( 17 ) located between said narrow end and said one electrode positioned near said narrow end; and
a size cut-off membrane ( 27 ) positioned above the capture means ( 17 ) capable of separating non-digested proteins from peptides, or larger size peptides from smaller size peptides.
2. The device of claim 1 , wherein the capture means are adapted for use in MALDI-MS or MALDI-TOF.
3. The device of claim 1 , wherein the capture means ( 17 ) is an immobilization support capable of capturing positively and/or negatively charged peptides.
4. The device of claim 1 , wherein the funnel shaped cavity ( 33 ) of the electroconcentration means ( 23 ) is in a position wherein said cavity is located in an essentially vertical position, the wide end being in a higher position than the narrow end.
5. A device for protein and/or peptide concentration in a sample, which device comprises
means for electroconcentrating ( 23 ) including a funnel shaped cavity ( 33 ) with a wide end and a narrow end;
at least two electrodes ( 7 , 35 ), one electrode ( 35 ) being positioned near to said wide end and one electrode ( 7 ) being positioned nearer to said narrow end;
two means for capturing proteins and/or peptides ( 17 ) located between said narrow end and said one electrode positioned near said narrow end: and said two capture means ( 67 A, 67 B) separated from each other, one of which is preceded by a mask allowing unmodified peptides to pass through and the other one of which allowing modified peptides to pass through.
6. The device of claim 5 , wherein the two capture means ( 17 ) are immobilization support capable of capturing positively and/or negatively charged peptides.
7. The device of claim 5 , wherein the funnel shaped cavity ( 33 ) of the electroconcentration means ( 23 ) is in a position wherein said cavity is located in an essentially vertical position, the wide end being in a higher position than the narrow end.
8. The device of claim 5 , wherein the capture means are adapted for use in MALDI-MS or MALDI-TOF.