Polynucleotide encoding lysyl-tRNA synthetase from
View Patent ↗This invention relates to isolated nucleic acid fragments encoding aminoacyl-tRNA synthetases and particulary lsoleucyl-, lysyl-, phenylalanyl- and prolyl-tRNA synthetases from Zea mays , Oryza sativa, Glycine max and Tritium aestivum. The invention also relates to the construction of a chimeric gene encoding all or a portion of the aminoacyl-tRNA synthetase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the aminoacyl-tRNA synthetase in a transformed host cell.
1. An isolated polynucleotide comprising:
(a) a nucleotide sequence encoding a polypeptide having lysyl-tRNA synthetase activity, wherein the polypeptide has an amino acid sequence of at least 90% sequence identity, based on the Clustal alignment method, when compared to SEQ ID NO:10; or
(b) a full-length complement of the nucleotide sequence of (a).
2. The polynucleotide of claim 1 , wherein the polypeptide has an amino acid sequence of at least 95% sequence identity, based on the Clustal alignment method, when compared to SEQ ID NO:10.
3. The polynucleotide of claim 1 , wherein the nucleotide sequence comprises SEQ ID NO:9.
4. The polynucleotide of claim 1 , wherein the amino acid sequence of the polypeptide comprises SEQ ID NO:10.
5. A chimeric gene comprising a polynucleotide of claim 1 , operably linked to a regulatory sequence.
6. A vector comprising a polynucleotide of claim 1 .
7. A method for transforming a cell comprising transforming a cell with a polynucleotide of claim 1 .
8. A cell comprising a chimeric gene of claim 5 .
9. A method for producing a plant comprising transforming a plant cell with a chimeric gene of claim 5 , and regenerating a plant from the transformed plant cell.
10. A plant comprising a chimeric gene of claim 5 .
11. A seed comprising a chimeric gene of claim 5 .