Essential downstream component of the wingless signaling pathway and therapeutic and diagnostic applications based thereon
An isolated polypeptide comprising a peptide which consists of a legless homology domain (HD); wherein the peptide inhibits Lgs function in colon cancer cells; and a composition comprising the same, are disclosed.
1. An isolated polypeptide comprising:
(i) a peptide consisting of amino acids 177 to 204 of SEQ ID NO:15, or
(ii) a peptide consisting of amino acids 349 to 383 of SEQ ID NO:15,
wherein said isolated polypeptide does not comprise both of said peptide (i) and (ii), and said isolated polypeptide inhibits tcf-driven luciferase activity in colon cancer cells.
2. A chimeric molecule comprising the polypeptide of claim 1 fused to a heterologous polypeptide.
3. The chimeric molecule according to claim 2 , wherein said heterologous polypeptide is selected from the group consisting of an antigenic epitope, glutathione-S-transferase, thioredoxin, and antibody.
4. A composition comprising the polypeptide of claim 1 , and a pharmaceutically acceptable carrier.