Genes for controlling floral development in orchid
The present invention provides PeMADS2, PeMADS3, PeMADS4 and PeMADS5 for controlling floral development in orchid. The present invention also provides a protein, a vector, a cell, a protocorn, and a kit for controlling the floral development in orchid. A method for producing transgenic orchid and a transgenic orchid are provided.
1. An isolated nucleic acid molecule that affects floral development in orchid, which nucleic acid molecule is selected from the group consisting of:
(a) a nucleic acid molecule, PeMADS2, comprising the nucleotide sequence of SEQ ID NO:1; and
(b) a nucleic acid molecule comprising degenerate sequence of SEQ ID NO:1 which encodes the PeMADS protein of SEQ ID NO:2.
2. A vector comprising the nucleic acid molecule according to claim 1 .
3. The vector according to claim 2 , which is a shuttle vector that is capable of expressing the nucleic acid molecule in a plant.
4. The vector according to claim 2 comprising an inducible promoter.
5. A kit for controlling floral development in orchid, which comprises the vector according to claim 2 .
6. An isolated cell transformed with the vector according to claim 2 .
7. A transgenic orchid comprising cells which contain the nucleic acid molecule according to claim 1 .
8. A transgenic orchid produced by transforming an orchid with the vector of claim 2 .
9. The cell according to claim 6 , wherein the cell is a prokaryote cell.
10. The cell according to claim 6 , wherein the cell is an orchid cell.
11. The cell according to claim 6 , wherein the cell is a Phalaenopsis spp. cell.
12. A method for producing a transformed orchid cell comprising introducing the nucleic acid molecule according to claim 1 into an orchid cell to obtain the orchid transformed cell.
13. The method according to claim 12 , wherein the orchid is a Phalaenopsis spp.
14. The method according to claim 12 , wherein the orchid cell is derived from a protocom-like body.
15. The method according to claim 12 , wherein introducing the nucleic acid molecule into the orchid cell is by a gene gun.
16. A protocorn-like body comprising a vector comprising the nucleic acid molecule according to claim 1 .
17. A method for producing a transgenic orchid comprising the steps of:
(a) introducing the nucleic acid molecule according to claim 1 into an orchid cell to obtain an orchid transformed cell; and
(b) regenerating the orchid transformed cell to obtain the transgenic orchid plant.
18. The method according to claim 17 , wherein the orchid plant is a Phalaenopsis spp.
19. The method according to claim 17 , wherein the orchid cell is derived from a protocorn-like body.
20. The method according to claim 17 , wherein the nucleic acid molecule is introduced into the orchid cell in step (a) by a gene gun.
21. A transgenic orchid produced according to the method according to claim 17 .
22. A method for controlling floral development in orchid, which comprises transforming an orchid plant with a nucleic acid molecule according to claim 1 , said nucleic acid molecule encoding a protein that is expressed in said plant.
23. The method according to claim 22 , wherein a gene gun is used to introduce the nucleic acid molecule into the cell.
24. The method according to claim 23 , wherein the cell is derived from a protocom-like body.
25. The isolated nucleic acid molecule according to claim 1 , which consists of the nucleotide sequence of SEQ ID NO:1.