Controlled release preparation of insulin and its method
A controlled release preparation of insulin and its method are provided. The controlled release preparation of insulin contains microparticles obtained by microencapsulation of uniform microcystals of insulin using biodegradable polymeric materials. Since the denaturation of insulin that may occur during microencapsulation is reduced, the stability of the preparation can be increased. Also, the ratio of insulin to a polymer carrier is increased, which is suitable for pulmonary delivery. Further, the controlled release preparation of insulin can continuously exhibit pharmaceutical efficacy in vivo in a stable manner for an extended period of time.
1. A controlled release preparation of insulin containing microparticles having a volume average diameter of 10 μm or less obtained by microencapsulation of insulin microcrystals having a volume average diameter which is less than the volume average diameter of the microparticles,
wherein the insulin microcrystals are formed by varying the pH of an acidic insulin solution, which is at an isoelectric point or below, up to within the range 9 to 10.5 to form insulin seeds that can act as nuclei of crystal; and lowering the solubility of insulin in the solution by lowering the pH of the solution,
wherein the microencapsulation is performed by double emulsion method comprising suspending the insulin microcrystals in a solution of pH 4.5 to 6.5, which is. an isoelectric precipitation zone; adding the suspended solution to a biodegradable polymer solution to make a primary emulsion; adding an emulsifier to the primary emulsion to make a secondary emulsion; and stirring the secondary emulsion to form microparticles.
2. The controlled release preparation of insulin containing microparticles according to claim 1 , wherein the microparticles are preserved in an isoelectric buffered solution of pH 4.5 to 6.5.
3. The controlled release preparation of insulin containing microparticles according to claim 1 or 2 , wherein the biodegradable polymer is selected from the group consisting of albumin, gelatin, collagen, fibrinogen, polylactides(PLA), polyglycolides (PGA), poly (lactide-co-glycolide)s (PLGA), polyethylene glycol (PEG), poly β-hydroxy butyric acid (PHB), polycaprolactone, polyanhydrides, polyorthoesters, polyurethanes, poly(butyric acid)s, poly(valeric acid)s, poly(lactide-co-caprolactone) and derivatives thereof, and copolymers thereof.
4. The controlled release preparation of insulin containing microparticles according to claim 3 , wherein the polymer is a poly(lactide-co-glycolide)s (PLGA).
5. The controlled release preparation of insulin containing microparticles according to claim 1 or 2 , wherein the solution for suspending the insulin crystals is one selected from the group consisting of a chitosan solution produced from a 1% acetate solution, a phosphate buffered saline (PBS) solution and supernatant solution obtained after centrifugation of insulin microcrystal solution.
6. The controlled release preparation of insulin containing microparticales according to claim 1 or 2 , wherein the preparation has one of formations for pulmonary inhaled administration, injection, oral administration and transdermal suction.
7. The controlled release preparation of insulin containing microparticles according to claim 1 or 2 , further comprising pharmaceutically acceptable diluents, carriers and additives.
8. The controlled release preparation of insulin containing microparticles according to claim 1 or 2 , wherein an immediately active insulin is also included.
9. A method of producing a controlled release preparation comprising the steps of:
(a) varying the pH of an acidic insulin solution, which is at an isoelectric point or below, up to within the range 9 to 10.5 to form insulin seeds that can act as nuclei of crystal; and lowering the solubility of insulin in the solution by lowering the pH of the solution to form insulin microcrystals,
(b) suspending the insulin microcrystals in a solution of pH 4.5 to 6.5, which is an isoelectric precipitation zone; adding the suspended solution to a biodegradable polymer solution to make a primary emulsion; adding an emulsifier to the primary emulsion to make a secondary emulsion; and stirring the secondary emulsion to form microparticles having a volume average diameter of 10 μm or less and greater than a volume average diameter of the microcrystals,
(c) preparing formulation by a general pharmaceutical method by adding pharmaceutically acceptable supportive ingredients or additives to the microparticles.
10. The method according to claim 9 , wherein the step (c) includes the step of preserving the microparticles in an isoelectric buffered solution of pH 4.5 to 6.5.
11. The method according to claim 9 or 10 , wherein the emulsifier is one of polyvinylaichol (PVA), polyvinylpirrolidone, alginates, methylcellulose and gelatine.
12. The method according to claim 9 or 10 , wherein the solution for suspending the insulin crystals is one selected from the group consisting of a chitosan solution produced from a 1% acetate solution, a phosphate buffered saline (PBS) solution and asupematant solution obtained after centrifugation of insulin microcrystal solution.