Internal ribosome entry site of the labial gene for protein expression
The invention describes compositions and methods for recombinant protein expression in a wide range of cell types. The compositions comprise an IRES sequence from the Drosophila labial (lab) gene, or a variant or fragment thereof, or alternatively, a homolog of a lab IRES, or a variant or fragment thereof. Methods of using the compositions are also described.
1. A nucleic acid vector for the expression of at least two cistrons comprising:
a. a promoter operably linked to a nucleotide sequence comprising at least two cistrons; and
b. a nucleotide sequence that provides IRES activity operably linked to each cistron subsequent to the first cistron, wherein at least one of the nucleotide sequences that provide IRES activity comprises a nucleotide sequence chosen from:
a nucleotide sequence comprising SEQ ID NO. 1;
a nucleotide sequence comprising nucleotides 1–215 of SEQ ID NO. 1;
a nucleotide sequence comprising nucleotides 45–239 of SEQ ID NO. 1;
a nucleotide sequence comprising nucleotides 45–215 of SEQ ID NO. 1;
a nucleotide sequence comprising nucleotides 1–74 and 187–239 of SEQ ID NO. 1;
a nucleotide sequence comprising nucleotides 1–74 and 187–215 of SEQ ID NO. 1;
a nucleotide sequence that differs from a nucleotide sequence comprising SEQ ID NO. 1 by substitution of the nucleotides at positions 124–127 of SEQ ID NO. 1;
a nucleotide sequence comprising SEQ ID NO. 2;
a nucleotide sequence that differs from a nucleotide sequence comprising SEQ ID NO. 2 by substitution of the nucleotides at positions 136–139 of SEQ ID NO. 2; and
a nucleotide sequence that differs from a nucleotide sequence comprising SEQ ID NO. 2 by substitution of the nucleotides at positions 126–129 of SEQ ID NO. 2.
2. The nucleic acid vector of claim 1 , wherein at least one of said at least two cistrons comprises a reporter gene.
3. The nucleic acid vector of claim 1 , wherein at least one of said at least two cistrons comprises a therapeutic gene.
4. A biological vector capable of expressing at least two cistrons comprising the nucleic acid vector of claim 1 .
5. The biological vector of claim 4 , wherein said biological vector is selected from poxvirus, adenovirus, herpesvirus, adeno-associated virus, retrovirus, and baculovirus.
6. A host cell comprising the nucleic acid vector of claim 1 .
7. The host cell of claim 6 , wherein said host cell is an insect cell.
8. The host cell of claim 7 , wherein said insect cell is a Drosophila cell.
9. A method for expressing at least two cistrons comprising: introducing into a host cell a nucleic acid vector comprising:
a. a promoter operably linked to a nucleotide sequence comprising at least two cistrons; and
b. a nucleotide sequence that provides IRES activity operably linked to each cistron subsequent to the first cistron, wherein at least one of the nucleotide sequences that provide IRES activity comprises a nucleotide sequence chosen from:
a nucleotide sequence comprising SEQ ID NO. 1;
a nucleotide sequence comprising nucleotides 1–215 of SEQ ID NO. 1;
a nucleotide sequence comprising nucleotides 45–239 of SEQ ID NO. 1;
a nucleotide sequence comprising nucleotides 45–215 of SEQ ID NO. 1;
a nucleotide sequence comprising nucleotides 1–74 and 187–239 of SEQ ID NO. 1;
a nucleotide sequence comprising nucleotides 1–74 and 187–215 of SEQ ID NO. 1;
a nucleotide sequence that differs from a nucleotide sequence comprising SEQ ID NO. 1 by substitution of the nucleotides at positions 124–127 of SEQ ID NO. 1;
a nucleotide sequence comprising SEQ ID NO. 2;
a nucleotide sequence that differs from a nucleotide sequence comprising SEQ ID NO. 2 by substitution of the nucleotides at positions 136–139 of SEQ ID NO. 2; and
a nucleotide sequence that differs from a nucleotide sequence comprising SEQ ID NO. 2 by substitution of the nucleotides at positions 126–129 of SEQ ID NO. 2.
10. A baculovirus transfer vector for the expression of at least two cistrons comprising:
a. a polyhedrin promoter operably linked to a nucleotide sequence comprising at least two cistrons; and
b. a nucleotide sequence that provides IRES activity operably linked to each cistron subsequent to the first cistron, wherein at least one of the nucleotide sequences that provide IRES activity comprises a nucleotide sequence chosen from:
a nucleotide sequence comprising SEQ ID NO. 1;
a nucleotide sequence comprising nucleotides 1–215 of SEQ ID NO. 1;
a nucleotide sequence comprising nucleotides 45–239 of SEQ ID NO. 1;
a nucleotide sequence comprising nucleotides 45–215 of SEQ ID NO. 1;
a nucleotide sequence comprising nucleotides 1–74 and 187–239 of SEQ ID NO. 1;
a nucleotide sequence comprising nucleotides 1–74 and 187–215 of SEQ ID NO. 1;
a nucleotide sequence that differs from a nucleotide sequence comprising SEQ ID NO. 1 by substitution of the nucleotides at positions 124–127 of SEQ ID NO. 1;
a nucleotide sequence comprising SEQ ID NO. 2;
a nucleotide sequence that differs from a nucleotide sequence comprising SEQ ID NO. 2 by substitution of the nucleotides at positions 136–139 of SEQ ID NO. 2; and
a nucleotide sequence that differs from a nucleotide sequence comprising SEQ ID NO. 2 by substitution of the nucleotides at positions 126–129 of SEQ ID NO. 2.
11. The baculovirus transfer vector of claim 10 , wherein at least one of at least two cistrons comprises a reporter gene.
12. The baculovirus transfer vector of claim 10 , wherein at least one of at least two cistrons comprises a therapeutic gene.
13. A recombinant baculovirus capable of expressing at least two cistrons in a host cell comprising a baculovirus genome comprising:
a. a polyhedrin promoter operably linked to a nucleotide sequence comprising at least two cistrons; and
b. a nucleotide sequence that provides IRES activity operably linked to each cistron subseauent to the first cistron, wherein at least one of the nucleotide sequences that provide IRES activity comprises a nucleotide sequence chosen from:
a nucleotide sequence comprising SEQ ID NO. 1;
a nucleotide sequence comprising nucleotides 1–215 of SEQ ID NO. 1;
a nucleotide sequence comprising nucleotides 45–239 of SEQ ID NO. 1;
a nucleotide sequence comprising nucleotides 45–215 of SEQ ID NO. 1;
a nucleotide sequence comprising nucleotides 1–74 and 187–239 of SEQ ID NO. 1;
a nucleotide sequence comprising nucleotides 1–74 and 187–215 of SEQ ID NO. 1;
a nucleotide sequence that differs from a nucleotide sequence comprising SEQ ID NO. 1 by substitution of the nucleotides at positions 124–127 of SEQ ID NO. 1;
a nucleotide sequence comprising SEQ ID NO. 2;
a nucleotide sequence that differs from a nucleotide sequence comprising SEQ ID NO. 2 by substitution of the nucleotides at positions 136–139 of SEQ ID NO.2; and
a nucleotide sequence that differs from a nucleotide sequence comprising SEQ ID NO. 2 by substitution of the nucleotides at positions 126–129 of SEQ ID NO. 2.
14. An in vitro method for producing a recombinant baculovirus capable of expressing at least two cistrons comprising:
a. introducing a baculovirus transfer vector of claim 10 and a baculovirus genomic DNA into a baculovirus host cell so as to effect homologous recombination; and
b. isolating a recombinant baculovirus.
15. The method of claim 14 , wherein said recombinant baculovirus is isolated by selecting plaques expressing at least one of said at least two cistrons.
16. A baculovirus host cell expressing at least two cistrons comprising the recombinant baculovirus of claim 13 .