Process for producing isoprenoid compounds by microorganisms and a method for screening compounds with antibiotic or weeding activity
The present invention provides a process for producing isoprenoid compounds or proteins encoded by DNA using DNA that contains one or more of the DNA encoding proteins having activity to improve efficiency in the biosynthesis of isoprenoid compounds effective in pharmaceuticals for cardiac diseases, osteoporosis, homeostasis, prevention of cancer, and immunopotentiation, health food and anti-fouling paint products against barnacles; the DNA; the protein; and a method for screening a substance with antibiotic and weeding activities comprising screening a substance inhibiting enzymatic reaction on the non-mevalonate pathway.
1. A process for producing an isoprenoid compound comprising the steps of:
introducing a vector containing DNA encoding a protein comprising an amino acid sequence of SEQ ID NO:5 into a prokaryotic host cell to produce a transformant;
culturing the transformant in a medium;
allowing the transformant to produce and accumulate the isoprenoid compound; and
recovering the isoprenoid compound.
2. A process for producing an isoprenoid compound comprising the steps of:
culturing a prokaryotic transformant harboring a vector containing DNA encoding a protein comprising an amino acid sequence of SEQ ID NO:5 in a medium;
allowing the transformant to produce and accumulate the isoprenoid compound; and
recovering the isoprenoid compound.
3. The process according to claim 1 or 2 , wherein the DNA comprises a nucleotide sequence of SEQ ID NO:10.
4. The process according to claim 1 or 2 , wherein the isoprenoid compound is selected from the group consisting of ubiquinone, vitamin K 2 and carotenoid.
5. The process according to claim 3 , wherein the isoprenoid compound is selected from the group consisting of ubiquinone, vitamin K 2 and carotenoid.
6. A process for producing an isoprenoid compound comprising the steps of:
introducing a vector containing DNA which hybridizes with a nucleotide sequence consisting of SEQ ID NO:10 in the presence of 0.7 to 1.0 mol/l NaCl at 65° C. followed by washing in a 0.1 to 2-fold SSC solution at 65° C. and encodes a protein having activity to catalyze a reaction to produce 2-C-methyl-D-erythritol 4-phosphate from 1-deoxy-D-xylulose 5-phosphate into a prokaryotic host cell to produce a transformant;
culturing the transformant in a medium;
allowing the transformant to produce and accumulate the isoprenoid compound; and
recovering the isoprenoid compound.
7. A process for producing an isoprenoid compound comprising the steps of:
culturing a prokaryotic transformant harboring a vector containing DNA that hybridizes with a nucleotide sequence consisting of SEQ ID NO:10 in the presence of 0.7 to 1.0 mol/l NaCl at 65° C. followed by washing in a 0.1 to 2-fold SSC solution at 65° C. and encodes a protein having activity to catalyze a reaction to produce 2-C-methyl-D-erythritol 4-phosphate from 1-deoxy-D-xylulose 5-phosphate in a medium;
allowing the transformant to produce and accumulate the isoprenoid compound; and
recovering the isoprenoid compound.
8. The process according to claim 6 or 7 , wherein the isoprenoid compound is selected from the group consisting of ubiquinone, vitamin K 2 and carotenoid.