Compositions and methods for optimized hybridization using modified solutions
The invention provides a composition, kit and method for hybridizing a probe and target at a temperature lower than their standard hybridization temperature. The chemical component added to the composition has a formula R(NH 2 )C═O, where R is amino or alkyl. A method for use of the chemical component and composition is also disclosed.
1. A method that allows a probe and target to hybridize at a temperature lower than their standard hybridization temperature, comprising:
(a) adding to the probe and target a chemical component of the formula:
R(NH 2 )C═O
where R is an alkyl group having 1–6 carbon atoms;
(b) heating the probe and target in the presence of the added component; and
(c) allowing the probe and target to hybridize.
2. A method as recited in claim 1 , wherein said probe and target are heated to at least their standard hybridization temperature.
3. A method as recited in claim 1 , wherein said chemical component is added to a solution.
4. A method that allows a probe on a micro array surface to hybridize to a target at a temperature lower than their standard hybridization temperature, comprising:
(a) adding to the probe and target a chemical component of the formula:
R(NH 12 )C═O where R is an alkyl group;
(b) heating the probe and target in the presence of the added component; and
(c) allowing the probe and target to hybridize.
5. A method as recited in claim 4 , wherein said alkyl group has 1–6 carbon atoms.
6. A method as recited in claim 4 , wherein said probe and target are heated to at least their standard hybridization temperature.
7. A method as recited in claim 4 , wherein said chemical component is added to a solution.
8. The method of claim 1 , wherein said component is acetamide.
9. The method of claim 4 , wherein said chemical component is acetamide.