Haemostatically active preparation containing vWF and method for the production thereof

1. A process for preparing a hemostatically active preparation containing von Willebrand factor (vWF) from a fraction of human plasma by

a) chromatographic purification of a vWF-containing plasma fraction on an anion-exchange material which has the anion-exchanging groups on grafted polymeric structures (tentacle materials), collecting a vWF-containing fraction, characterized in that

b) purification of said fraction using gel permeation to prepare a purified thermally stable vWF-containing preparation follows, and

c) heating the preparation for 1–5 hours (h) at a temperature of more than 80° C. in a lyophilized state for inactivating viruses and is stable in an aqueous solution at room temperature for at least one week.

2. The process according to claim 1 , characterized in that the thermally stable preparation and the heated preparation exhibit only one peak in HPLC gel filtration analysis.

3. The process according to claim 1 , characterized in that the preparation is heated at a temperature of more than 90° C.

4. The process according to claim 1 , characterized in that the preparation is heated at a temperature of 100° C.

5. The process according to claim 1 , characterized in that the preparation is heated for 2–3 h.

6. The process according to claim 1 , characterized in that said purification by gel permeation is effective as a final measure for protein purification.

7. The process according to claim 1 , characterized in that said fraction is purified from pooled human plasma.