Methods of detecting interactions between proteins, peptides or libraries thereof using fusion proteins
The present invention provides a method for identifying a polypeptide that interacts with a known protein, which method uses fusion proteins with GFP fragments.
1. A first and a second fusion protein,
said first fusion protein comprising a polypeptide linked to a first Green Fluorescent Protein (GFP) fragment,
said second fusion protein comprising a polypeptide linked to a second GFP fragment,
wherein a full length GFP is dissected between the amino acid residues of a surface loop to generate said first and second GFP fragments, and
wherein association of the first and second GFP fragments results in a GFP that exhibits detectable fluorescence.
2. The first and a second fusion protein according to claim 1 , wherein the first GFP fragment is dissected from the second GFP fragment between amino acid residues 157 and 158 of the full length GFP.