Thermal strip thermocycler
View Patent ↗This is a self-contained disposable thermal cycler device in which target sequence is amplified as the samples passes over a grid of alternating temperature and then at the completion of the reaction, the amplified material is captured by probes complementary to the targeted sequence. Since the device can be sealed and is disposable, it reduces the occurrence of cross contamination or specimen carry-over.
1. A thermal strip thermocycler comprising:
a substrate,
a rectangular porous membrane, overlaying the substrate and enclosed by a clear impermeable non-electrically conductive material and having a first end and a second end opposing the first end,
a conductive circuit printed on a surface of the substrate,
the conductive circuit having parallel alternating zones of temperature produced by resistive heating elements positioned along the conductive circuit, and positioned such that a long axis and direction of flow within the porous membrane is perpendicular to the parallel alternating zones of temperature created by the conductive circuit,
a sample addition pad on the first end of and in contact with the porous membrane,
a fluid absorption pad on the opposing second end of and in contact with the porous membrane, and
a heat sink placed on a side of the porous membrane opposing the conductive circuit.
2. A thermal strip thermocycler according to claim 1 wherein: the porous membrane has reagents necessary for an enzymatic or chemical reaction dried onto it.
3. A thermal strip thermocycler according to claim 1 wherein: the sample addition pad has reagents necessary for an enzymatic or chemical reaction dried onto it.
4. A thermal strip thermocycler according to claim 1 wherein: the heat sink comprises a cooling bar.
5. A thermal strip thermocycler according to claim 1 wherein: the heat sink has ridges, and wherein contact of the ridges with the at least one of the substrate and the non-electrically conductive material creates an intercalating pattern relative to the conductive circuit.
6. A thermal strip thermocycler according to claim 1 wherein: the heat sink has an access for attachment of temperature sensing devices for measuring the temperature at various areas of the porous membrane.
7. A thermal strip thermocycler according to claim 1 wherein: the thermal strip thermocycler is enclosed in a case and wherein the case comprises a top face with openings for sample addition over the sample addition pad, a vent hole proximate the fluid absorption pad, a thermocouple contact for thermally monitoring a reaction, and a transparent viewing port for optically monitoring an amplification reaction.
8. A thermal strip thermocycler according to claim 1 wherein: the thermal strip thermocycler is enclosed in a case made of a clear material for optically monitoring an amplification reaction therethrough.
9. A thermal strip thermocycler according to claim 1 wherein: the conductive circuit comprises first and second conductive circuits for providing a desired temperature in an initial cycle of thermocycling.
10. A thermal strip thermocycler according to claim 1 wherein: adjacent segments of the conductive circuit have varying width dimensions.
11. A thermal strip thermocycler according to claim 10 wherein: an initial segment of the conductive circuit is narrower than adjacent segments of a sinuous pattern thereof to provide a higher temperature for an initial cycle of thermocycling.
12. A thermal strip thermocycler according to claim 1 wherein: the conductive circuit includes at least two resistive circuits powered by different voltage sources and printed in an interlacing sinuous pattern allowing for additional temperature control.
13. A thermal strip thermocycler according to claim 12 further comprising: an additional circuit to provide a higher temperature for an initial cycle of thermocycling.
14. A thermal strip thermocycler according to claim 1 wherein: the substrate comprises at least two low resistance circuits printed in an intermeshing comb pattern on a surface thereof.
15. A thermal strip thermocycler according to claim 14 further comprising: a closer spacing for a first tine of the intermeshing comb pattern of the at least two low resistance circuits to provide less electrical resistance and a higher temperature for an initial cycle of thermocycling.
16. A thermal strip thermocycler according to claim 1 wherein: said the sample pad is heated by a separate conductive circuit with a separate voltage source.
17. A thermal strip thermocycler according to claim 1 wherein: a closeable gap separates the sample addition pad from the porous membrane such that sample can be incubated at a given time before flow of sample and reagents onto the porous membrane is initiated and flow can be initiated by sliding the sample addition pad and membrane either manually or by action of an instrument.
18. A thermal strip thermocycler comprising: a substrate,
a rectangular porous membrane, overlaying the substrate and enclosed by a clear impermeable non-electrically conductive material and having a first end and a second end opposing the first end,
a conductive circuit printed on a surface of the substrate,
the conductive circuit having parallel alternating zones of temperature produced by resistive heating elements positioned along the conductive circuit, and positioned such that a long axis and direction of flow within the porous membrane is perpendicular to the parallel alternating zones of temperature created by the conductive circuit,
wherein adjacent segments of the conductive circuit have varying width dimensions, and wherein an initial segment of the conductive circuit is narrower than adjacent segments of a sinuous pattern thereof to provide a higher temperature for an initial cycle of thermocycling,
a sample addition pad on the first end of and in contact with the porous membrane, and
a fluid absorption pad on the second opposing end of and in contact with the porous membrane.
19. A thermal strip thermocycler comprising:
a substrate,
a rectangular porous membrane, overlaying the substrate and enclosed by a clear impermeable non-electrically conductive material and having a first end and a second end opposing the first end,
a conductive circuit printed on a surface of the substrate,
the conductive circuit having parallel alternating zones of temperature produced by resistive heating elements positioned along the conductive circuit, and positioned such that a long axis and direction of flow within the porous membrane is perpendicular to the parallel alternating zones of temperature created by the conductive circuit,
wherein the conductive circuit includes at least two resistive circuits powered by different voltage sources and printed in an interlacing sinuous pattern allowing for additional temperature control,
a sample addition pad on the first end of and in contact with the porous membrane, and
a fluid absorption pad on the opposing second end of and in contact with the porous membrane.
20. A thermal strip thermocycler according to claim 19 further comprising: an additional circuit to provide a higher temperature for an initial cycle of thermocycling.
21. A thermal strip thermocycler comprising:
a substrate comprising at least two low resistance conductive circuits printed in an intermeshing comb pattern on a surface thereof,
a rectangular porous membrane, overlaying the substrate and enclosed by a clear impermeable non-electrically conductive material and having a first end and a second end opposing the first end,
the conductive circuits having parallel alternating zones of temperature produced by resistive heating elements positioned along the conductive circuits and positioned such that a long axis and direction of flow within the porous membrane is perpendicular to the parallel alternating zones of temperature created by the conductive circuit,
a sample addition pad on the first end of and in contact with the porous membrane, and
a fluid absorption pad on the opposing second end of and in contact with the porous membrane.
22. A thermal strip thermocycler comprising:
a substrate comprising at least two low resistance circuits printed in an intermeshing comb pattern on a surface thereof with a closer spacing for a first tine of the intermeshing comb pattern of the at least two low resistance circuits to provide less electrical resistance and a higher temperature for an initial cycle of thermocycling,
a rectangular porous membrane, overlaying the substrate and enclosed by a clear impermeable non-electrically conductive material and having a first end and a second end opposing the first end,
the conductive circuits having parallel alternating zones of temperature produced by resistive heating elements positioned along the conductive circuits and positioned such that a long axis and direction of flow within the porous membrane is perpendicular to the parallel alternating zones of temperature created by the conductive circuit,
a sample addition pad on the first end of and in contact with the porous membrane, and
a fluid absorption pad on the opposing second end of and in contact with the porous membrane.
23. A thermal strip thermocycler comprising:
a substrate,
a rectangular porous membrane, overlaying the substrate and enclosed by a clear impermeable non-electrically conductive material and having a first end and a second end opposing the first end,
a conductive circuit printed on a surface of the substrate,
the conductive circuit having parallel alternating zones of temperature produced by resistive heating elements positioned along the conductive circuit, and positioned such that a long axis and direction of flow within the porous membrane is perpendicular to the parallel alternating zones of temperature created by the conductive circuit,
a sample addition pad on the first end of and in contact with the porous membrane, wherein a closeable gap separates the sample addition pad from the rectangular porous membrane such that sample can be incubated at a given time before flow of sample and reagents onto the porous membrane is initiated and flow can be initiated by sliding the sample addition pad and membrane either manually or by action of an instrument, and
a fluid absorption pad on the opposing second end of and in contact with the porous membrane.