Lactoferricin gene and transformant expressing lactoferricin
Disclosed is a gene encoding a mutated lactoferricin peptide and an E. coli cell transformed with the same, wherein the mutated lactoferricin peptide has substitutions at the 7th and 10th amino acid residues of the wildtype bovine lactoferricin. The gene of the present invention can be expressed in E. coli at high efficiency, and the expressed mutated lactoferricin peptide can be purified at high yield, thus making its mass production possible.
1. A mutated lactoferricin peptide having the amino acid sequence set forth in SEQ ID NO: 3, wherein glutamate and valine substituted for Gln7 and Met10, respectively, of the wildtype bovine lactoferricin, wherein said mutated lactoferricin retains the antimicrobial activity of wild type lactoferrin.
2. An isolated gene encoding the mutated lactoferricin peptide of claim 1 , wherein said gene comprises the nucleotide sequence set forth in SEQ ID NO: 2.
3. A recombinant expression vector pET-MLnX comprising a fusion gene containing the gene of claim 2 and a magainin gene, wherein the fusion gene comprises the nucleotide sequence set forth in SEQ ID NO: 4.
4. The recombinant expression vector pET-MLnX as set forth in claim 3 , wherein the recombinant expression vector is selected from the group consisting of a pET-ML1X plasmid, a pET-ML2X plasmid, a pET-ML3X plasmid and a pET-ML4X plasmid, which comprise tandem repeats of 1, 2, 3 and 4 copies of the fusion gene, respectively.
5. An E. coli cell (BL21(DE3)[pET-MLnX]) transformed with the recombinant expression vector pET-MLnX of claim 3 or 4 .
6. A method of producing the lactoferricin peptide of claim 1 , comprising the steps of:
culturing the E. coli cell (BL21(DE3)[pET-MLnX]) of claim 5 ;
treating the resulting incubated E. coli cell with IPTG;
harvesting the culture; and
recovering the peptide from the culture.