Purging of cells using viruses
View Patent ↗The subject invention relates to viruses that are able to purge (reduce or eliminate) undesirable cells in a mixture of cells. Undesirable cells can include neoplastic cells, cells mediating graft-versus host diseases, and autoimmune cells. The subject invention also relates to the purging of undesirable cells from bone marrow or peripheral blood cell harvests in the treatment of mammals including cancer patients, transplant recipients, and patients with autoimmune disease.
1. A method of reducing or eliminating neoplastic cells in an ex vivo mixture of normal, hematopoeitic cells and neoplastic cells comprising contacting said mixture with a vesicular stomatitis virus, wherein the mixture is a suspension.
2. A method as in claim 1 wherein said neoplastic cells are leukemia cells.
3. A method as in claim 1 wherein said hematopoeitic cells are marrow cells.
4. A method as in claim 1 wherein said hematopoeitic cells are peripheral blood cells.
5. A method as in claim 1 wherein said virus is replication-competent.
6. A method as in claim 1 wherein said neoplastic cells are lymphoma cells.
7. A method as in claim 1 further comprising administering a chemotherapeutic agent to the mixture before, during or after contacting with said virus.
8. A method of treating cancer in a mammal comprising:
a) removing bone marrow or peripheral blood cells from said mammal as a suspension,
b) incubating said suspension of bone marrow or peripheral blood cells ex vivo with an effective amount of a vesicular stomatitis virus to yield purged bone marrow or peripheral blood cells,
c) performing myeloablative treatment on said mammal, and
d) transplanting into said mammal the purged bone marrow or peripheral blood cells of step b.
9. A method as in claim 7 wherein said myeloablative treatment is high dose chemotherapy.
10. A method of treating cancer in a mammal receiving a transplant of bone marrow or peripheral blood stem cells comprising purging said bone marrow or peripheral blood stem cells by incubating a suspension of said bone marrow or peripheral blood stem cells ex vivo with an effective amount of a vesicular stomatitis virus, and administering the resulting purged cells to said mammal.