Capillary electrophoretic apparatus, sample plate and sample injection method
An electrode plate of a sample plate is set on the body of an electrophoretic apparatus, while a plug is inserted into a migration high voltage line connection hole and connected to a high-tension distribution cable. Each well of a base plate is inserted into a through hole of a well guide and further press-fit and engaged into a cavity of an electrode plate, for fixing the base plate to the electrode plate. Thereafter a sample is introduced into each well of the base plate and an end of a capillary column is dipped into each well for applying a migration voltage and electrophoretically injecting the sample into the capillary column.
1. A sample injection method for a capillary electrophoretic apparatus comprising a migration part electrophoresing a sample injected into an end of a capillary column charged with a gel of a separation carrier and detection means detecting each component separated in the capillary column on an appropriate position of the capillary column, the sample injection method comprising the steps of:
providing a preliminary separation carrier for preliminary separation between the sample and a capillary column sample injection end,
injecting the sample into the capillary column through the preliminary separation carrier, and
removing the preliminary separation carrier before starting the electrophoresis.
2. A capillary electrophoretic apparatus comprising:
a capillary column charged with a gel of a separation carrier;
a migration part for electrophoresing a sample injected into an end of said capillary column;
detection means for detecting each component separated in the capillary column on an appropriate position of the capillary column; and
a preliminary separation part for storing a preliminary separation carrier for preliminary separation and the sample so that the preliminary separation carrier is located between a sample injection end of the capillary column and the sample,
wherein the sample is set in the preliminary separation carrier in a state adsorbed by a filter medium.
3. The capillary electrophoretic apparatus in accordance with claim 2 , wherein the preliminary separation part comprises a fluid gel as the preliminary separation carrier.
4. The capillary electrophoretic apparatus in accordance with claim 2 , wherein the migration part is a multi-capillary array migration part including a plurality of capillary columns so arranged that a plurality of samples are injected one by one into the capillary columns and simultaneously electrophoresed in all the capillary columns.
5. A capillary electrophoretic apparatus comprising:
a capillary column charged with a gel of a separation carrier;
a migration part for electrophoresing a sample injected into an end of said capillary column;
detection means for detecting each component separated in the capillary column on an appropriate position of the capillary column; and
a preliminary separation part for storing a preliminary separation carrier for preliminary separation and the sample so that the preliminary separation carrier is located between a sample injection end of the capillary column and the sample,
wherein the preliminary separation part comprises a fluid gel as the preliminary separation carrier and is so stratified that an aqueous solution containing the sample forms an upper layer and the preliminary separation carrier forms a lower layer, and the sample injection end of the capillary column is inserted into the preliminary separation carrier.
6. The capillary electrophoretic apparatus in accordance with claim 5 , wherein the migration part is a multi-capillary array migration part including a plurality of capillary columns so arranged that a plurality of samples are injected one by one into the capillary columns and simultaneously electrophoresed in all the capillary columns.