Peptidyl t-RNA hydrolase (PTH)
The invention provides pth polypeptides and polynucleotides encoding pth polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing pth polypeptides to screen for antibacterial compounds.
1. An isolated polypeptide selected from the group consisting of:
(i) an isolated polypeptide comprising the amino acid sequence of SEQ ID NO:2 or 4;
(ii) an isolated polypeptide which is the amino acid sequence of SEQ ID NO:2 or 4; and
(iii) a polypeptide which is encoded by a recombinant polynucleotide comprising the polynucleotide sequence of SEQ ID NO:1 or 3.
2. An isolated polypeptide selected from the group consisting of:
(a) an isolated polypeptide which comprises the amino acid of SEQ ID NO:4;
(b) an isolated polypeptide which is the polypeptide of SEQ ID NO:4; and
(c) an isolated polypeptide which is encoded by a polynucleotide comprising the sequence contained in SEQ ID NO:3.