Production of GM-CSF in plants
View Patent ↗The present invention discloses a method of producing granulocyte-macrophage colony stimulating factor (GM-CSF) in a plant comprising, transforming the plant with a genetic construct comprising a regulatory region functional in the plant, operably associated with a GM-CSF coding sequence, or a fragment or a derivative thereof, operably associated with a transcriptional terminator, and expressing the GM-CSF. Also disclosed are transgenic plants, seeds and cells comprising GM-CSF coding sequences and plant optimized GM-CSF coding sequences.
1. A method of producing granulocyte-macrophage colony stimulating factor (GM-CSF) in a cereal crop comprising growing a cereal crop that has a stably integrated genetic construct that comprises a glutelin regulatory region operably associated with a GM-CSF coding sequence as set forth in SEQ ID NO: 1, or a fragment thereof that retains GM-CSF activity of supporting proliferation of TF-1 cells, operably associated with a transcriptional terminator.
2. The method according to claim 1 , wherein the cereal crop is selected from the group consisting of: rice, wheat, oats, rye, corn, sorghum, and barley.
3. The method according to claim 1 , wherein the GM-CSF coding sequence encodes an N-terminal methionine residue.
4. The method according to claim 2 , wherein the cereal crop is rice.
5. The method according to claim 1 , wherein the GM-CSF coding sequence is operably linked to a signal sequence.
6. The method according to claim 1 , wherein the GM-CSF coding sequence is SEQ ID NO:1.
7. A transgenic cereal crop plant comprising a stably integrated genetic construct that comprises a glutelin regulatory region operably associated with a GM-CSF coding sequence as set forth in SEQ ID NO:1, or a fragment thereof that retains GM-CSF activity of supporting proliferation of TF-1 cells, operably associated with a transcriptional terminator.
8. The transgenic cereal crop according to claim 7 , wherein the cereal crop is selected from the group consisting of: rice, wheat, oats, rye, corn, sorghum, and barley.
9. The transgenic cereal crop according to claim 7 , wherein the GM-CSF coding sequence encodes an N-terminal methionine residue.
10. The transgenic cereal crop according to claim 8 , wherein the cereal crop is rice, japonica cultivar.
11. The transgenic cereal crop according to claim 7 , wherein the GM-CSF coding sequence is operably linked to a signal sequence.
12. The transgenic cereal crop according to claim 7 , wherein the GM-CSF coding sequence is SEQ ID NO:1.
13. A genetic construct comprising a glutelin regulatory region operably associated with a GM-CSF coding sequence as set forth in SEQ ID NO:1, or a fragment thereof that retains GM-CSF activity of supporting proliferation of TF- 1 cells, operably associated with a transcriptional terminator.
14. The genetic construct according to claim 13 , wherein the cereal crop is selected from the group consisting of: rice, wheat, oats, rye, corn, sorghum, and barley.
15. The genetic construct according to claim 13 , wherein the GM-CSF coding sequence encodes an N-terminal methionine residue.
16. The genetic construct according to claim 14 , wherein the cereal crop is rice, japonica cultivar.
17. The genetic construct according to claim 13 , wherein the GM-CSF coding sequence is operably linked to a signal sequence.
18. The genetic construct according to claim 13 , wherein the GM-CSF coding sequence is SEQ ID NO:1.
19. An isolated nucleotide sequence comprising the sequence set-forth in SEQ IDNO:1.
20. A DNA vector comprising the genetic construct of claim 13 .
21. A DNA vector comprising the isolated nucleotide sequence of claim 19 .
22. A transgenic cereal crop plant comprising the genetic construct of claim 13 .
23. A transgenic cereal crop plant comprising the isolated nucleotide sequence of claim 19 .