Compositions for identification and isolation of stem cells
The invention provides monoclonal antibodies that selectively bind to ectodermally- and endodermally-derived stem cells and methods for the diagnosis of a neoplasm in a subject by contacting a tissue sample from the subject with the antibodies. Also disclosed are methods for isolating such stem cells from a heterogeneous cell population by contacting the population with antibodies which selectively bind to stem cells.
1. An isolated monoclonal antibody produced by a hybridoma deposited with the American Type Culture Collection as Accession No. PTA-4655, or an antigen-binding fragment thereof.
2. The isolated antibody, or antigen-binding fragment thereof, of claim 1 , wherein the antigen-binding fragment thereof is selected from the group consisting of an F ab fragment, an F (ab′)2 fragment, and an Fv fragment.
3. The isolated antibody or antigen-binding fragment thereof of claim 1 , wherein the isolated antibody, or antigen-binding fragment thereof, is coupled with a detectable substance.
4. The isolated antibody, or antigen-binding fragment thereof, of claim 3 , wherein the detectable substance is selected from the group consisting of enzymes, prosthetic groups, fluorescent materials, luminescent materials, bioluminescent materials, and radioactive materials.
5. The isolated antibody, or antigen-binding fragment thereof, of claim 4 , wherein the enzyme is selected from the group consisting of horseradish peroxidase, alkaline phosphatase, β-galactosidase, and acetylcholinesterase.
6. The isolated antibody, or antigen-binding fragment thereof, of claim 4 , wherein the prosthetic group is selected from the group consisting of streptavidin, biotin, and avidin.
7. The isolated antibody, or antigen-binding fragment thereof, of claim 4 , wherein the fluorescent material is selected from the group consisting of umbelliferone, fluorescein, fluorescein isothiocyanate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride, and phycoerythrin.
8. The isolated antibody, or antigen-binding fragment thereof, of claim 4 , wherein the luminescent material is luminol.
9. The isolated antibody, or antigen-binding fragment thereof, of claim 4 , wherein the bioluminescent material is selected from the group consisting of luciferase, luciferin, and aequorin.
10. The isolated antibody, or antigen-binding fragment thereof, of claim 4 , wherein the radioactive material is selected from the group consisting of 125 I, 131 I, 35 S, and 3 H.
11. The isolated antibody, or antigen-binding fragment thereof, of claim 1 , wherein the isolated antibody, or antigen-binding fragment thereof, is coupled with a cytotoxic agent.
12. A diagnostic kit comprising the isolated antibody, or antigen-binding fragment thereof, of claim 1 .
13. A diagnostic kit comprising the isolated antibody, or antigen-binding fragment thereof, of claim 2 .
14. A diagnostic kit comprising the isolated antibody, or antigen-binding fragment thereof, of claim 5 .
15. An isolated recombinant antibody, or an antigen-binding fragment thereof, comprising all antigen binding specificities of an antibody produced by a hybridoma deposited with the American Type Culture Collection as Accession No. PTA-4655.
16. The isolated recombinant antibody, or an antigen-binding fragment thereof, of claim 15 , wherein the recombinant antibody is a chimeric antibody or a humanized antibody.
17. A diagnostic kit comprising the isolated antibody, or antigen-binding fragment thereof, of claim 15 .
18. A diagnostic kit comprising the isolated antibody, or antigen-binding fragment thereof, of claim 16 .