Isolated nucleic acid encoding L-lysine: 2-oxoglutaric acid 6-aminotransferase
Provided are an isolated gene capable of participating in the production of L-homoglutamic acid, and a production system of L-homoglutamic acid by using this gene. The gene is derived from the genome of Flavobacterium lutescens.
1. An isolated nucleic acid sequence selected from the group consisting of:
(a) the nucleotide sequence of SEQ ID NO: 1 encoding a protein having L-lysine: 2-oxoglutaric acid 6-aminotransferase activity, or the full complement thereof;
(b) a nucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO: 11 which has L-lysine: 2-oxoglutaric acid 6-aminotransferase activity, or the full complement thereof;
(c) a nucleotide sequence consisting of nucleotides 545 to 2658 of SEQ ID NO: 1 encoding a protein having L-lysine: 2-oxoglutaric acid 6-aminotransferase activity, or the full complement thereof;
(d) a nucleotide sequence consisting of nucleotides 801 to 2276 of SEQ ID NO: 1 encoding a protein having L-lysine: 2-oxoglutaric acid 6-aminotransferase activity, or the full complement thereof;
(e) a nucleotide sequence which has at least 95% homology with the nucleotide sequence of (c) or (d) encoding a protein having L-lysine: 2-oxoglutaric acid 6-aminotransferase activity, or the full complement thereof;
(f) a fragment of nucleotide sequence (a) or (b) encoding a protein having L-lysine: 2-oxoglutaric acid 6-aminotransferase activity, or the full complement thereof; and
(g) a nucleotide sequence which hybridizes under stringent conditions at 60° C. in 0.2×SSC to sequence (a), (b), (c), (d), or (f) and encodes a protein having L-lysine: 2-oxoglutaric acid 6-aminotransferase activity.
2. The isolated nucleotide sequence according to claim 1 , which is the nucleotide sequence of SEQ ID NO: 1 encoding a protein having L-lysine: 2-oxoglutaric acid 6-aminotransferase activity, or the full complement thereof.
3. The isolated nucleotide sequence according to claim 1 , which is the nucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO: 11 which has L-lysine: 2-oxoglutaric acid 6-aminotransferase activity, or the full complement thereof.
4. The isolated nucleotide sequence according to claim 1 , which is the nucleotide sequence consisting of nucleotides 545 to 2658 of SEQ ID NO: 1 encoding a protein having L-lysine: 2-oxoglutaric acid 6-aminotransferase activity, or the full complement thereof.
5. The isolated nucleotide sequence according to claim 1 , which is the nucleotide sequence consisting of nucleotides 801 to 2276 of SEQ ID NO: 1 encoding a protein having L-lysine: 2-oxoglutaric acid 6-aminotransferase activity, or the full complement thereof.
6. The isolated nucleotide sequence according to claim 1 , which is the nucleotide sequence which has at least 95% homology with the nucleotide sequence of (c) or (d) encoding a protein having L-lysine: 2-oxoglutaric acid 6-aminotransferase activity, or the full complement thereof.
7. The isolated nucleotide sequence according to claim 1 , which is the fragment of nucleotide sequence (a) or (b) encoding a protein having L-lysine: 2oxoglutaric acid 6-aminotransferase activity, or the full complement thereof.
8. The isolated nucleotide sequence according to claim 1 , which is the nucleotide sequence which hybridizes under stringent conditions at 60° C. in 0.2×SSC to sequence (a), (b), (c), (d), or (f) and encodes a protein having L-lysine 2-oxoglutaric acid 6-aminotransferase activity.
9. The isolated nucleotide sequence according to claim 1 , which is obtained from a bacterium belonging to Flavobacterium lutescens.
10. A nucleic acid construct comprising the nucleic acid sequence according to claim 1 .
11. The nucleic acid construct according to claim 10 , which is contained in Flavobacterium lutescens IFO 3084 (pCF213) deposited under accession number FERM BP-6797.
12. An isolated host cell comprising the nucleic acid construct according to claim 10 , wherein the nucleic acid sequence encodes a protein having L-lysine: 2-oxoglutaric acid 6-aminotransferase activity.
13. A process for producing L-homoglutamic acid, which comprises culturing the host cell according to claim 12 under suitable conditions to produce the protein in the presence of 1-piperidine-6-carboxylic acid, and recovering L-homoglutamic acid.
14. The process according to claim 13 , wherein the host cell is a bacterium belonging to the genus Flavobacterium.
15. The process according to claim 13 , wherein the host cell is Flavobacterium lutescens IFO 3084 (pCF213) deposited under accession number FERM BP-6797.