Glutamate transporter associated proteins and methods of use thereof
Glutamate Transporter Associated Proteins and nucleotide encoding Glutamate Transporter Associated Proteins are provided. Also provided is a method for identifying a compound that modulates a cellular response mediated by a Glutamate Transporter Associated Protein. A method is further provided for identifying a compound that inhibits an interaction between a Glutamate Transporter Associated Protein and a glutamate transporter protein. A method is provided for treating a disorder associated with glutamate transport.
1. An isolated polynucleotide selected from the group consisting of:
(a) a polynucleotide encoding a polypeptide having the amino acid sequence as set forth in SEQ ID NO:6;
(b) a polynucleotide of (a), wherein all T's are replaced by U's;
(c) a polynucleotide complementary to the full length polynucleotide of (a); and
(d) a polynucleotide having the nucleotide sequence as set forth in SEQ ID NO:5.
2. An expression vector comprising a polynucleotide of claim 1 .
3. The expression vector of claim 2 , wherein the vector is virus-derived.
4. The expression vector of claim 2 , wherein the vector is plasmid-derived.
5. An isolated host cell comprising a vector of claim 2 .
6. A method for producing a polypeptide comprising the steps of:
(a) culturing a host cell of claim 5 under conditions suitable for the expression of the polypeptide; and
(b) recovering the polypeptide from the host cell culture.
7. The isolated polynucleotide of claim 1 , wherein the polynucleotide encodes a polypeptide having the amino acid sequence as set forth in SEQ ID NO:6.
8. The isolated polynucleotide of claim 1 , wherein the polynucleotide has the nucleotide sequence as set forth in SEQ ID NO:5.