Oral compositions for white blood cell activation and proliferation
The present invention relates to health food, health drink, and health supplement. The invention relates to biological compositions comprising yeast cells that can improve the immune functions of animals. The invention also relates to methods for manufacturing the biological compositions, and methods of using the biological compositions to produce a healthful benefit in a subject with immunodeficiency disorders or immunosuppression by promoting activation and proliferation of white blood cells.
1. A biological composition comprising activated yeast cells, wherein said activated yeast cells are cells of Saccharomyces cerevisiae Hansen strain IFFI1202 and are characterized by their ability to increase the number of white blood cells in an immunodeficient or immunosuppressed subject as compared to yeast cells of said strain not having been so activated, wherein said activated yeast cells are prepared by using yeast cells of Saccharomyces cerevisiae Hansen strain IFFI1202 in a method comprising in any order the steps of:
(a) culturing said yeast cells in a first electromagnetic field or series of electromagnetic fields having a frequency in the range of 9,921 to 9,930 MHz and a field strength in the range of 220 to 240 mV/cm;
(b) culturing said yeast cells in a second electromagnetic field or series of electromagnetic fields having a frequency in the range of 10,121 to 10,130 MHz and a field strength in the range of 225 to 245 mV/cm;
(c) culturing said yeast cells in a third electromagnetic field or series of electromagnetic fields having a frequency in the range of 12,141 to 12,150 MHz and a field strength in the range of 235 to 255 mV/cm;
(d) culturing said yeast cells in a fourth electromagnetic field or series of electromagnetic fields having a frequency in the range of 12,831 to 12,840 MHz and a field strength in the range of 270 to 290 mV/cm; and
(e) culturing said yeast cells in a fifth electromagnetic field or series of electromagnetic fields having a frequency in the range of 12,871 to 12,880 MHz and a field strength in the range of 250 to 270 mV/cm.
2. The biological composition of claim 1 , wherein the activated yeast cells are at a concentration of about 10 3 cells per ml.
3. The biological composition of claim 1 , wherein the activated yeast cells are dried.
4. A method for preparing a biological composition comprising activated yeast cells, wherein said activated yeast cells are cells of Saccharomyces cerevisiae Hansen strain IFFI1202 and are characterized by their ability to increase the number of white blood cells in an immunodeficient or immunosuppressed subject as compared to yeast cells of said strain not having been so activated, said method comprising providing yeast cells of Saccharomyces cerevisiae Hansen strain IFFI1202, and in any order the steps of:
(a) culturing said yeast cells in a first electromagnetic field or series of electromagnetic fields having a frequency in the range of 9,921 to 9,930 MHz and a field strength in the range of 220 to 240 mV/cm;
(b) culturing said yeast cells in a second electromagnetic field or series of electromagnetic fields having a frequency in the range of 10,121 to 10,130 MHz and a field strength in the range of 225 to 245 mV/cm;
(C) culturing said yeast cells in a third electromagnetic field or series of electromagnetic fields having a frequency in the range of 12,141 to 12,150 MHz and a field strength in the range of 235 to 255 mV/cm;
(d) culturing said yeast cells in a fourth electromagnetic field or series of electromagnetic fields having a frequency in the range of 12,831 to 12,840 MHz and a field strength in the range of 270 to 290 mV/cm; and
(e) culturing said yeast cells in a fifth electromagnetic field or series of electromagnetic fields having a frequency in the range of 12,871 to 12,880 MHz and a field strength in the range of 250 to 270 mV/cm.