Biomedical devices with antimicrobial coatings
Biomedical devices with antimicrobial coatings are provided. One or more surfaces of the device are coated with a cationic peptide, cationic proteins, or mixtures thereof to impart antimicrobial properties to the surface.
1. A biomedical device at least one surface of which comprises a coating effective amount of at least one synthetic antimicrobial peptide comprising three segments A, B and C, in any order, wherein
segment A is a peptide having the sequence SEQ ID NO. 1;
segment B is a peptide having the sequence SEQ ID NO. 2; and
segment C is a linking group of up to 10 amino acids, and which does not inhibit the antimicrobial activity of the peptide or induce toxicity in mammalian cells, and which includes 0 to about 10 amino acids.
2. The device of claim 1 wherein said device is selected from the group consisting of catheters, implants, stents, fluid collection bags, sensors, hydrogel bandages, tubing, carriers for antibiotic, diagnostic and therapeutic agents, and ophthalmic devices.
3. The device of claim 1 wherein the device is an ophthalmic device.
4. The device of claim 1 wherein segment C has a formula —HN—(CR 1 R 2 ) n —CO—wherein n is an integer between 1 and 21, R 1 and R 2 are independently selected from the group consisting of H, straight or branched alkyl groups having 1 to 4 carbon atoms, straight or branched hydroxyl groups having 1–2 carbon atoms, straight or branched alkylthio groups having 1 to 3 carbon atoms, carbamoyl groups having 1 to 3 carbon atoms, carboxy groups having 1 to 3 carbon atoms, primary and secondary amino groups having 1 to 4 carbon atoms and 1 to 3 nitrogen atoms, benzyl, phenol, phenyl indoles and N,N-pyrroles.
5. The device of claim 4 wherein n is an integer between 1 and 10 and at least one of R 1 and R 2 is H.
6. The device of claim 1 , wherein the A and B segments are in terminal positions and segment C comprises up to 5 amino acids.
7. The device of claim 1 , wherein the at least one surface comprises a coating effective amount of melimine, protattin and mixtures thereof.
8. The device of claim 7 , wherein said melimine is L-melimine.
9. The device of claim 7 , wherein said melimine is D-melimine.
10. The device of claim 1 , wherein the surface further comprises a polymer selected from the group consisting of hydrogels, silicone hydrogels, polymers and copolymers of 2-hydroxyethylmethacrylate and mixtures thereof.
11. The device of claim 10 wherein the polymer is a hydrogel.
12. The device of claim 10 wherein the polymer is a silicone hydrogel.
13. The device of claim 10 wherein the polymer is a polymer or copolymer of 2-hydroxyethylmethacrylate.
14. The device of claim 13 wherein the copolymer of 2-hydroxyethylmethacrylate is a lightly crosslinked copolymer of 2-hydroxyethylmethacrylate.
15. The device of any of claims 1 to 3 or 4 – 14 , wherein said device is a contact lens.