Metalloprotease highly expressed in the testis, MMP-29
The present invention provides novel polynucleotides encoding MMP-29 polypeptides, fragments and homologues thereof. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing said polypeptides. The invention further relates to diagnostic and therapeutic methods for applying these novel MMP-29 polypeptides to the diagnosis, treatment, and/or prevention of various diseases and/or disorders related to these polypeptides. The invention further relates to screening methods for identifying agonists and antagonists of the polynucleotides and polypeptides of the present invention.
1. An isolated polypeptide comprising a polypeptide sequence selected from the group consisting of:
(a) an isolated polypeptide comprising amino acids 1 to 569 of SEQ ID NO:2;
(b) an isolated polypeptide comprising amino acids 2 to 569 of SEQ ID NO:2; and
(c) an isolated polypeptide comprising amino acids 25 to 569 of SEQ ID NO:2; wherein said polypeptide has metalloproteinase activity.
2. The isolated polypeptide of claim 1 , wherein said polypeptide is (a).
3. The isolated polypeptide of claim 1 , wherein said polypeptide is (b).
4. The isolated polypeptide of claim 1 , wherein said polypeptide is (c).
5. The isolated polypeptide of claim 1 wherein said polypeptide sequence further comprises a heterologous polypeptide.
6. The isolated polypeptide of claim 5 wherein said heterologous polypeptide is the Fc domain of immunoglobulin.
7. An isolated polypeptide produced by a method comprising:
(a) culturing an isolated recombinant host cell comprising a vector comprising the coding region encoding the polypeptide of claim 1 under conditions such that the polypeptide of claim 1 is expressed; and
(b) recovering said polypeptide.
8. An isolated polypeptide comprising a polypeptide having a polypeptide sequence that is at least 99.7% identical to amino acids 2 to 569 of SEQ ID NO:2, wherein percent identity is calculated using a CLUSTALW global sequence alignment, and wherein said polypeptide has metalloproteinase activity.
9. The isolated polypeptide according to claim 8 , wherein said polypeptide contains a single amino acid substitution.