Group 2 allergen specific IgE-Fabs and use thereof
The present invention relates to group 2 allergen specific IgE-Fabs and whole Ig molecules as well as use thereof. More precisely, the present invention relates to grass pollen specific IgE-Fabs (Phl p2) and reagents, kits and vaccines comprising these. The invention also relates to use of group 2 specific IgE-Fabs and whole Ig for, inter alia, diagnosis, therapy and prevention of type I allergy.
1. A grass pollen group 2 allergen specific human IgE Fab having a heavy chain consisting of the amino acid sequence as shown in SEQ ID NO: 7, SEQ ID NO: 8, or SEQ ID NO: 9, and a light chain consisting of the amino acid sequence as shown in SEQ ID NO: 10, SEQ ID NO: 11, or SEQ ID NO: 12.
2. A grass pollen group 2 allergen specific human IgE Fab having a heavy chain consisting of the amino acid sequence as shown in SEQ ID NO: 7, SEQ ID NO: 8, or SEQ ID NO: 9, and a light chain consisting of the amino acid sequence as shown in SEQ ID NO: 10, SEQ ID NO: 11, or SEQ ID NO: 12, respectively.
3. An isolated grass pollen group 2 allergen specific antibody comprising the variable regions of the IgE Fab of claim 2 grafted onto human IgG1.
4. A grass pollen group 2 allergen specific human IgE Fab having a heavy chain encoded by the nucleic acid sequence as shown in SEQ ID NO: 1, SEQ ID NO: 2, or SEQ ID NO: 3, and a light chain encoded by the nucleic acid as shown in SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO: 6, respectively.
5. The antibody according to claim 3 , wherein the antibody is directed against Phl p 2.
6. A diagnostic reagent comprising the antibody according to claim 3 .
7. A diagnostic kit comprising the reagent according to claim 6 .
8. The IgE Fab according to claim 2 , wherein the IgE Fab is directed against Phl p 2.
9. The IgE Fab according to claim 2 , wherein the IgE Fab is recombinantly produced.
10. A diagnostic reagent comprising the IgE Fab according to claim 2 .
11. A diagnostic kit comprising the reagent according to claim 10 .
12. A method for diagnosing type I allergy, comprising contacting a sample with the IgE Fab according to claim 2 .
13. A method for environmental allergen detection, comprising contacting a sample with the IgE Fab according to claim 2 .
14. A method for standardization of allergen extract, comprising standardizing the allergen extract with the IgE Fab according to claim 2 .
15. A grass pollen group 2 allergen specific human IgE Fab having a heavy chain encoded by the nucleic acid sequence as shown in SEQ ID NO: 1, SEQ ID NO: 2, or SEQ ID NO: 3, and a light chain encoded by the nucleic acid as shown in SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO: 6.
16. An isolated grass pollen group 2 allergen specific antibody comprising the variable regions of the IgE Fab of claim 4 grafted onto human IgG1.
17. The IgE Fab according to claim 4 , wherein the IgE Fab is directed against Phl p 2.
18. A diagnostic reagent comprising the IgE Fab according to claim 4 .
19. A diagnostic kit comprising the reagent according to claim 18 .