Plant fatty acid synthases and use in improved methods for production of medium-chain fatty acids
By this invention, compositions and methods of use related to β-ketoacyl-ACP synthase Of special interest are synthases obtainable from Cuphea species. Amino acid and nucleic acid for synthase protein factors are provided, as well as methods to utilize such sequences in constructs for production of genetically engineered plants having altered fatty acid compositions. Of particular interest is the expression of synthase protein factors in conjunction with expression of plant medium-chain acyl-ACP thioesterases for production of increased levels and/or modified ratios of medium-chain fatty acids in oils of transgenic plant seeds.
1. A method for producing medium-chain fatty acids in transgenic plant seeds by expression of one or more plant medium-chain thioesterase proteins heterologous to said transgenic plant, comprising
(a) providing for expression of a plant synthase factor protein heterologous to said transgenic plant, wherein said synthase is a β-ketoacyl-ACP synthase (KAS) factor A protein from a Cuphea species:
(b) providing for expression of said one or more plant medium-chain thioesterase proteins in conjunction with (a),
whereby the percentage of medium-chain fatty acids produced in said transgenic plant seeds expressing both said plant synthase factor protein and said one or more plant medium-chain thioesterase proteins is increased as compared to the percentage of medium-chain fatty acids produced in seeds expressing only said one or more plant medium-chain thioesterase proteins.
2. The method of claim 1 , wherein said Cuphea species is C. hookeriana or C. pulcherrima.
3. The method of claim 2 wherein said KAS factor A protein comprises a coding sequence as set forth in SEQ ID NO:5.
4. The method of claim 2 wherein said KAS factor A protein comprises a coding sequence encoding an amino acid sequence as set forth in SEQ ID NO:6.
5. The method of claim 2 wherein said synthase factor A protein comprises a coding sequence encoding an amino acid sequence set forth in residues 125-466 of SEQ ID NO:6.