Identification and characterization of an Anthocyanin mutant (
The present invention is directed to a novel plant phenotype, designated Anthocyanin 1 (ANT1), a nucleic acid sequence expressed in plants demonstrating the ANT1 phenotype and the corresponding amino acid sequence. Also provided are plant cells and plants that exhibit modified ANT1 expression.
1. A transgenic plant, comprising a plant transformation vector comprising a nucleic acid sequence which encodes an ANT1 polypeptide having the amino acid sequence of SEQ ID NO: 2, wherein the plant has an ANT1 phenotype.
2. The transgenic plant of claim 1 , wherein the plant is tomato or tobacco.
3. The transgenic plant of claim 2 , wherein the transgenic plant is tomato, and wherein the tomato produces an anthocyanin selected from the group consisting of delphinidin 3-rutinoside-5-glucoside, delphinidin 3-(coumaroyl)rutinoside-5-glucoside, delphinidin 3-(caffeoyl)rutinoside-5-glucoside, petunidin 3-rutinoside-5-glucoside, petunidin 3-(coumaroyl)rutinoside-5-glucoside, petunidin 3-(caffeoyl)rutinoside-5-glucoside, malvidin3-rutinoside-5-glucoside, malvidin 3-(coumaroyl)rutinoside-5-glucoside, and malvidin 3-(caffeoyl)rutinoside-5-glucoside.
4. The transgenic plant of claim 2 , wherein the plant is tobacco, and wherein the tobacco produces an anthocyanin selected from the group consisting of cyaniding-3-glucoside and cyaniding-3-rutinoside.
5. A method of isolating an anthocyanin from a plant, said method comprising introducing into progenitor cells of the plant a plant transformation vector comprising a nucleic acid sequence which encodes an ANT1 polypeptide having at least 95% sequence identity to the amino acid sequence SEQ ID NO: 2, growing the transformed progenitor cells to produce a transgenic plant having an ANT1 phenotype that expresses the polynucleotide sequence comprised by the vector, and extracting the anthocyanin from the plant.
6. The plant of claim 5 , wherein the plant is tomato or tobacco.
7. The method of claim 5 , wherein the anthocyanin is selected from the group consisting of delphinidin 3-rutinoside-5-glucoside, delphinidin 3-(coumaroyl)rutinoside-5-glucoside, deiphinidin 3-(caffeoyl)rutinoside-5-glucoside, petunidin 3-(coumaroyl)rutinoside-5-glucoside, malvidin3-rutinoside-5-glucoside, and malvidin 3(caffeoyl)rutinoside-5-glucoside.
8. The method of claim 5 , wherein the nucleic acid sequence encodes an ANT1 polypeptide having the amino acid sequence of SEQ ID NO: 2.
9. A method of isolating glycetein from a plant, said method comprising introducing into progenitor cells of the plant a plant transformation vector comprising a nucleic acid sequence which encodes an ANT1 polypeptide having at least 95% sequence identity to the amino acid sequence SEQ ID NO: 2, growing the transformed progenitor cells to produce a transgenic plant having an ANT1 phenotype that expresses the polynucleotide sequence comprised by the vector, and extracting the glycetein from the plant.
10. The method of claim 9 , wherein the plant is tomato.
11. The method of claim 9 , wherein the nucleic acid sequence encodes an ANT1 polypeptide having the amino acid sequence of SEQ ID NO: 2.