Method of delivering a bioactive agent with porous β-tricalcium phosphate granules
A porous β-tricalcium phosphate material for bone implantation is provided. The multiple pores in the porous TCP body are separate discrete voids and are not interconnected. The pore size diameter is in the range of 20-500 μm, preferably 50-125 μm. The porous β-TCP material provides a carrier matrix for bioactive agents and can form a moldable putty composition upon the addition of a binder. Preferably, the bioactive agent is encapsulated in a biodegradable agent. The invention provides a kit and an implant device comprising the porous β-TCP, and a bioactive agent and a binder. The invention also provides an implantable prosthetic device comprising a prosthetic implant having a surface region, a porous β-TCP material disposed on the surface region and optionally comprising at least a bioactive agent or a binder. Methods of producing the porous β-TCP material and inducing bone formation are also provided.
1. A method of delivering a bioactive agent at a site in a mammal requiring bone formation, comprising implanting at the site a composition comprising
porous β-tricalcium phosphate (β-TCP) granules that have a particle size of 0.1-2 mm and that comprise a multiplicity of pores having a pore diameter size of 20-500 μm and being single separate voids partitioned by walls and being not interconnected and
a bioactive agent.
2. The method of claim 1 , wherein the bioactive agent is a bone morphogenic protein.
3. The method of claim 1 , wherein the bioactive agent is encapsulated in a biodegradable agent.
4. The method of claim 3 , wherein the delivery of the bioactive agent is sustained released.
5. The method of claim 1 , wherein the bioactive agent is a nucleic acid molecule comprising a sequence encoding a bone morphogenic protein.
6. The method of claim 3 , wherein the biodegradable agent is poly(lactide-co-glycolide) (PLGA).
7. The method of claim 1 , wherein the composition further comprises carboxy methylcellulose sodium.
8. The method of claim 1 , wherein the porous β-TCP granules have a particle size of 0.5-1 mm.
9. The method of claim 1 , wherein the pore diameter size is 50-125 μm.