Anti-VEGF antibodies
Humanized and variant anti-VEGF antibodies and various uses therefor are disclosed. The anti-VEGF antibodies have strong binding affinities for VEGF; inhibit VEGF-induced proliferation of endothelial cells in vitro; and inhibit tumor growth in vivo.
1. An isolated antibody that binds human VEGF comprising a heavy chain variable domain comprising SEQ ID NO: 126 as CDRH1, SEQ ID NO: 2 as CDRH2 and SEQ ID NO: 127 as CDRH3 and a light chain variable domain comprising SEQ ID NO: 4 as CDRL1, SEQ ID NO: 5 as CDRL2 and SEQ ID NO: 6 as CDRL3.
2. The isolated antibody of claim 1 , wherein the heavy chain variable domain comprises SEQ ID NO: 116 and the light chain variable domain comprises SEQ ID NO: 115.
3. An aqueous solution comprising the isolated antibody of claim 2 .
4. The aqueous solution of claim 3 comprising at least one of the following ingredients: an amino acid, a disaccharide and a non-ionic surfactant.
5. The aqueous solution of claim 4 comprising an amino acid, a disaccharide and a non-ionic surfactant.
6. The aqueous solution of claim 3 comprising at least one of the following ingredients: histidine, trehalose and a non-ionic surfactant.
7. The aqueous solution of claim 6 comprising histidine, trehalose and a non-ionic surfactant.