Polynucleotides encoding BSL2v1c2-Ig
The present invention provides nucleic acids encoding B7-related factors that modulate the activation of immune or inflammatory response cells, such as T-cells. Also provided are expression vectors and fusion constructs comprising nucleic acids encoding B7-related polypeptides, including BSL1, BSL2, and BSL3. The present invention further provides isolated B7-related polypeptides, isolated fusion proteins comprising B7-related polypeptides, and antibodies that are specifically reactive with B7-related polypeptides, or portions thereof. In addition, the present invention provides assays utilizing B7-related nucleic acids, polypeptides, or peptides. The present invention further provides compositions of B7-related nucleic acids, polypeptides, fusion proteins, or antibodies that are useful for the immunomodulation of a human or animal subject.
1. An isolated polynucleotide sequence comprising a nucleic acid selected from the group consisting of:
(a) an isolated polynucleotide sequence comprising a polynucleotide encoding the amino acids 1 to 480 of SEQ ID NO:133;
(b) an isolated polynucleotide sequence comprising a polynucleotide encoding the amino acids 2 to 480 of SEQ ID NO:133; and
(c) an isolated polynucleotide sequence comprising a polynucleotide encoding the amino acids 29 to 480 of SEQ ID NO:133.
2. The isolated polynucleotide sequence of claim 1 , wherein said polynucleotide is (a).
3. The isolated polynucleotide sequence of claim 2 , wherein said polynucleotide comprises nucleotides 1 to 1440 of SEQ ID NO:132.
4. The isolated polynucleotide of claim 1 , wherein said polynucleotide is (b).
5. The isolated polynucleotide sequence of claim 4 , wherein said polynucleotide comprises nucleotides 4 to 1440 of SEQ ID NO:132.
6. The isolated polynucleotide of claim 1 , wherein said polynucleotide is (c).
7. The isolated polynucleotide sequence of claim 6 , wherein said polynucleotide comprises nucleotides 85 to 1440 of SEQ ID NO:132.
8. An isolated polynucleotide sequence comprising the cDNA clone contained in plasmid BSL2v1c2Ig in ATCC Deposit No. PTA-4056.
9. An isolated recombinant vector comprising the isolated nucleic acid molecule of claim 1 .
10. An isolated recombinant host cell comprising the vector of claim 9 .
11. A method of making an isolated polypeptide comprising:
(a) culturing the recombinant host cell of claim 10 under conditions such that said polypeptide is expressed; and
(b) recovering said polypeptide.