IP Library › Granted Patent US 7,371,513
Granted Patent B2
US 7,371,513 · App. 11/015,180 · Granted May 13, 2008

Method of preserving corneal tissue using polyoxyethylene/polyoxypropylene copolymer

Assignee: Regents of the University of California
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Quick Facts
Patent No.
US 7,371,513
App. No.
11/015,180
Granted
May 13, 2008
Kind
B2
Abstract

Tissue preservation media comprising a polyoxyethylene/polyoxypropylene copolymer are used to preserve tissues and organs for storage and transplantation. In particular embodiments, the polyoxyethylene/polyoxypropylene copolymer is Pluronic F68 or FLOCOR (CRL-5861; purified poloxamer 188), and the medium is Steinhardt medium, polyoxyethylene/polyoxypropylene copolymer-supplemented Optisol GS or polyoxyethylene/polyoxypropylene copolymer-supplemented ViaSpan.

Claims (9)

1. A method of preserving tissue, comprising the step of:

incubating a tissue in a tissue preservation medium at 4 degrees C. for between 7 and 21 days, the medium containing a polyoxyethylene/polyoxypropylene copolymer in final concentration of 0.5 to 5 mg/ml, and the tissue is cornea tissue.

2. The method of claim 1 wherein the polyoxyethylene/polyoxypropylene copolymer is FLOCOR (CRL-5861; purified poloxamer 188).

3. The method of claim 1 wherein the polyoxyethylene/polyoxypropylene copolymer is Pluronic F68.

4. The method of claim 1 wherein the medium is Steinhardt mediums.

5. The method of claim 1 wherein the medium is Optisol GS supplemented with the polyoxyethylene/polyoxypropylene copolymer.

6. The method of claim 1 wherein the medium is ViaSpan supplemented with the polyoxyethylene/polyoxypropylene copolymer.

7. The method of claim 1 further comprising the step of verifying post-incubation survival and transplant utility of the tissue.

8. The method of claim 1 wherein the polyoxyethylene/polyoxypropylene copolymer is Pluronic F68, and the final concentration is 1 mg/ml.

Assignments (4)
CONFIRMATORY LICENSE Recorded Nov 8, 2022
From: UNIVERSITY OF CALIFORNIA BERKELEY
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 061897/0077 →
CONFIRMATORY LICENSE Recorded Sep 25, 2019
From: UNIVERSITY OF CALIFORNIA, BERKELEY
To: NIH
Reel/Frame 050490/0707 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 17, 2008
From: STEINHARDT, RICHARD A.
To: REGENTS OF THE UNIVERSITY OF CALIFORNIA
Reel/Frame 020378/0438 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 17, 2004
From: STEINHARDT, RICHARD A.
To: UNIVERSITY OF CALIFORNIA, THE
Reel/Frame 016109/0080 →
Continuity (2)
Continuation In Part 1073833100 · Dec 17, 2003
Related Publication 20050156378A1 · Jul 21, 2005