Multi-functional and configurable assay
View Patent ↗Novel magnetic assay methods and systems. According to a preferred embodiment, a chromatographic medium is provided that is designed to be contacted with a test solution having activated magnetic particles such that the solution flows bilaterally thereacross. A magnetic field, generated by a magnet or electromagnet, is selectively applied to the medium which causes the charged particles to become substantially bound at a site on the medium specified by the position of the magnet, to thus form a captured line or zone. In one preferred embodiment, the magnetic field is applied at the site on the medium at which the test solution is contacted. The assay is multi-configurable and can be modified to suit a particular immuno-separation procedure or application.
1. A multi-conformable assay system for use in performing a magnetic chromatography method, the assay comprising:
(a) a microscope slide;
(b) a housing positionable upon said microscope slide, said housing having a sample well formed thereon for receiving a reaction mixture having a quantity of magnetic particles suspended therein, said sample well tapered to have an opening;
(c) at least one absorbent member positionable within said housing and adjacent to said sample well to absorb a portion of said reaction mixture deposited in said sample well not containing said magnetic particles; and
(d) a layer of gel formed upon said microscope slide and aligned with said opening formed on said sample well, said layer of gel being operative to receive and contact said reaction mixture deposited through said sample well, wherein said gel comprises at least one digestive enzyme operative to react with a protein present within said reaction mixture.
2. The assay of claim 1 wherein said housing comprises a generally block-like structure defining an upper platform surface, said sample well being formed in the general midsection of said upper platform surface, said housing further defining first and second voids on opposed sides of said sample well; said system further comprising first and second absorbent pads, said first and second absorbent pads being received into dedicated ones of said voids of opposed sides of said sample well.
3. The assay of claim 2 wherein said housing is fabricated from a rigid, black material selected from the group consisting of plastic and metal.
4. The assay of claim 1 wherein said gel includes at least one receptor operative to bind with at least one of said magnetic particles of said reaction mixture.
5. The assay of claim 1 further comprising a magnet positionable below the microscope slide, wherein the magnet is alignable with said opening formed on said sample well.
6. A multi-conformable assay system for use in performing a magnetic chromatography method, the assay comprising:
(a) a microscope slide;
(b) a housing positionable upon said microscope slide, said housing having a sample well formed thereon for receiving a reaction mixture having a ciuantity of magnetic particles suspended therein, said sample well tapered to have an opening;
(c) at least one absorbent member positionable within said housing and adjacent to said sample well to absorb a portion of said reaction mixture deposited in said sample well not containing said magnetic particles;
(d) a layer of mesh formed upon said microscope slide, said layer of mesh defining a target area for receiving said reaction mixture as deposited through said sample well; and
(e) a layer of gel formed upon said mesh alignable with said opening formed on said sample well such that said reaction mixture is contacted with said gel and deposited through said sample well, wherein said gel comprises at least one digestive enzyme operative to react with a protein present within said reaction mixture.
7. The assay of claim 6 wherein said housing is detachable from said absorbent pads, mesh and said microscope slide.
8. The assay of claim 6 wherein said housing and said absorbent pads are removable from said mesh and said microscope slide.
9. The assay of claim 8 wherein said mesh is removable from said microscope slide base.
10. The assay of claim 6 wherein said housing comprises a generally block-like structure defining an upper platform surface, said sample well being formed in the general midsection of said upper platform surface, said housing further defining first and second voids on opposed sides of said sample well; said system further comprising first and second absorbent pads, said first and second absorbent pads being received into dedicated ones of said voids of opposed sides of said sample well.
11. The assay of claim 10 wherein said housing is fabricated from a rigid, black material selected from the group consisting of plastic and metal.
12. The assay of claim 6 wherein said gel includes at least one receptor operative to bind with at least one of said magnetic particles of said reaction mixture.
13. The assay of claim 6 further comprising a magnet positionable below the microscope slide, wherein the magnet is alignable with said opening on said sample well.